Calcium-dependent protein kinase 16 phosphorylates and activates the aquaporin PIP2;2 to regulate reversible flower opening in Gentiana scabra

第一作者：Keiichirou Nemoto

第一单位：日本岩手生物技术研究中心

通讯作者：Keiichirou Nemoto

 Abstarct 

背景回顾：Flower opening is important for successful pollination in many plant species, and some species repeatedly open and close their flowers. This is thought to be due to turgor pressure changes caused by water influx/efflux, which depends on osmotic oscillations in the cells. In some ornamental plants, water-transporting aquaporins, also known as plasma membrane intrinsic proteins (PIPs), may play an important role in flower opening. However, the molecular mechanism(s) involved in corolla movement are largely unknown. 

开花对许多植物的授粉成功十分重要，有些植物的花甚至会反复张开闭合。这被认为是由于水的内流/外流所引起的膨压变化，该过程取决于细胞内的渗透振荡。在一些观赏植物中，水通道蛋白，也被称为质膜内在蛋白（PIPs），可能在花朵张开的过程中发挥重要作用。然而，花冠运动的分子机制在很大程度上尚不清楚。

主要研究：Gentian (Gentiana spp.) flowers undergo reversible movement in response to temperature and light stimuli; using gentian as a model, we showed that the Gentiana scabra aquaporins GsPIP2;2 and GsPIP2;7 regulate repeated flower opening. 

龙胆属植物的花会响应于温度和光的刺激，发生可逆的运动；以龙胆草（Gentiana scabra）为模型，作者发现其水通道蛋白GsPIP2;2和GsPIP2;7控制龙胆花的反复张开。

结果：In particular, phosphorylation of a C-terminal serine residue of GsPIP2;2 is important for its transport activity and relates closely to the flower re-opening rate. Furthermore, GsPIP2;2 is phosphorylated and activated by the Ca²⁺-dependent protein kinase GsCPK16, which is activated by elevated cytosolic Ca2+ levels in response to temperature and light stimuli. 

GsPIP2;2此外，GsPIP2;2会受到钙依赖性蛋白激酶GsCPK16磷酸化和激活，而GsCPK16则会被龙胆响应温度和光刺激时升高的胞浆钙离子水平所激活。

结论：We propose that GsCPK16-dependent phosphorylation and activation of GsPIP2;2 regulate gentian flower re-opening, with stimulus-induced calcium signals acting as triggers.

综上，作者提出龙胆中依赖于GsCPK16的GsPIP2;2蛋白磷酸化和激活能够调控龙胆花的重新张开，而温度或光刺激诱导的钙信号是该过程的诱因。

doi: https://doi.org/10.1093/plcell/koac120

Journal: The Plant Cell

​Published date: April 20, 2022