Phosphate-induced resistance to pathogen infection in Arabidopsis

第一作者：Beatriz Val-Torregrosa

第一单位：西班牙巴塞罗那自治大学

第一通讯：Blanca San Segundo

 Abstarct 

背景回顾：In nature, plants are concurrently exposed to a number of abiotic and biotic stresses. 

在自然界中，植物会同时遭受各种生物和非生物胁迫。

提出问题：Our understanding of convergence points between responses to combined biotic/abiotic stress pathways remains, however, rudimentary. 

但是，目前我们对于植物响应生物/非生物复合胁迫途径的交汇点仍然了解较少。

结果1-高Pi与ROS：Here we show that MIR399 overexpression, loss-of-function of PHOSPHATE2 (PHO2), or treatment with high phosphate (Pi) levels is accompanied by an increase in Pi content and accumulation of reactive oxygen species (ROS) in Arabidopsis thaliana. 

本文中，作者发现拟南芥MIR399过表达、 PHO2基因功能缺失，或者高磷处理，通常伴随着植株Pi含量的增加以及活性氧物质（ROS）的积累。

结果2-高Pi与真菌抗性：High Pi plants (e.g., miR399 overexpressors, pho2 mutants, and plants grown under high Pi supply) exhibited resistance to infection by necrotrophic and hemibiotrophic fungal pathogens. 

通过上述不同方法获得的高Pi植株对于死体营养型和半活体营养型真菌的侵染具有一定程度的抗性。

结果3-高Pi与SA+JA：In the absence of pathogen infection, the expression levels of genes in the salicylic acid (SA)- and jasmonic acid (JA)-dependent signaling pathways were higher in high Pi plants compared to wild-type plants grown under control conditions, which is consistent with increased levels of SA and JA in non-infected high Pi plants. 

相比于野生型植株，在没有病原菌侵染的情况下，高Pi植株中水杨酸和茉莉酸依赖性信号通路上基因的表达水平要更高，这与未遭真菌侵染的高Pi植株中SA和JA水平升高结果相一致。

结果4-高Pi植物遭遇侵染时JA途径调控机制：During infection, an opposite regulation in the two branches of the JA pathway (ERF1/PDF1.2 and MYC2/VSP2) occurs in high Pi plants. Thus, while pathogen infection induces PDF1.2 expression in miR399 OE and pho2 plants, VSP2 expression is downregulated by pathogen infection in these plants. 

在真菌病原菌侵染时，高Pi植物中JA途径ERF1/PDF1.2和MYC2/VSP2两个分支上会发生相反的调控。因此，当病原菌侵染诱导miR399过表达和pho2突变体植株中PDF1.2基因的表达时，这些植株中VSP2基因的表达受到病原菌侵染的下调。

结论：This study supports the notion that Pi accumulation promotes resistance to infection by fungal pathogens in Arabidopsis, while providing a basis to better understand interactions between Pi signaling and hormonal signaling pathways for modulation of plant immune responses.

该研究表明植物中的Pi积累有助于促进其对于真菌病原菌侵染的抗性，为进一步理解植物免疫响应中的Pi信号转导和激素信号转导途径之间的互作提供新的视野。

doi: https://doi.org/10.1111/tpj.15680

Journal: the plant hjournal​

First Published: January 21, 2022