背景回顾：The interaction between chromatin and the nuclear lamina (NL) is intrinsically important to the establishment of three-dimensional chromatin architecture and spatiotemporal regulation of gene expression. 

提出问题：However, critical regulators involved in this process are poorly understood in plants. 

主要发现：Here, we report that Arabidopsis PNET2and its two homologs are bona fide inner nuclear membrane proteins and integral components of the NL. 

结果1-功能：PNET2s physically interact with the plant nucleoskeleton and engage nucleosome-enriched chromatin at the nuclear periphery. 

结果2-功能缺失的生长表型：Loss of all three PNET2s leads to severely disrupted growth and development, concomitant activation of abiotic and biotic stress responses, and ultimate lethality in Arabidopsis. 

结果3-功能缺失的分子表型：The pent2 triple mutant also displays drastic transcriptome changes accompanied by a globally altered chromatin architecture revealed by HiC analysis. 

结论：Our study identified PNET2 as an inner nuclear membrane (INM) component of the NL, which associates with chromatin and play a critical role in orchestrating gene expression and chromatin organization in plants.

 摘 要 

染色质与核纤层之间的互作对于建立染色质的三维结构以及基因表达的时空调控是非常重要的。但是，植物中参与该过程的重要调控子目前还不清楚。本文中，作者报道了拟南芥PNET2蛋白及其两个同源蛋白是内核膜蛋白，核纤层的组成组分。PNET2s与植物核骨架存在物理互作，在核周边区域吸引核小体富集的染色质。PNET2s三个蛋白功能全部丢失会导致严重的生长和发育缺陷，同时伴随着激活的生物和非生物胁迫响应，最终导致拟南芥死亡。pent2三突植株同时还表现出极为剧烈的转录组改变，伴随着全基因组范围的染色质结构改变。本文的研究结果揭示了PNET2蛋白作为一个核纤层的内核膜蛋白组分，与染色质相关，在调控植物的基因表达和染色质组织结构方面发挥重要作用。

 通讯作者 

** Yangnan Gu **