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Plant Biotechnol J:NF-YC14-PYR1模块整合ABA信号转导作用于大豆干旱耐受性

已有 617 次阅读 2021-10-5 00:52 |个人分类:每日摘要|系统分类:论文交流

The NF-Y-PYR module integrates the abscisic acid signal pathway to regulate plant stress tolerance

第一作者Tai-Fei Yu

第一单位中国农科院作物科学研究所

第一通讯You-Zhi Ma


 Abstract 


背景回顾Drought and salt stresses impose major constraints on soybean production worldwide. 


提出问题:However, improving agronomically valuable soybean traits under drought conditions can be challenging due to trait complexity and multiple factors that influence yield.


主要发现:Here, we identified a nuclear factor Y C subunit (NF-YC) family transcription factor member, GmNF-YC14, which formed a heterotrimer with GmNF-YA16 and GmNF-YB2 to activate the GmPYR1-mediated abscisic acid (ABA) signalling pathway to regulate stress tolerance in soybean. 


结果1-功能缺失突变体:Notably, we found that CRISPR/Cas9-generated GmNF-YC14 knockout mutants were more sensitive to drought than wild-type soybean plants. 


结果2-过表达:Furthermore, field trials showed that overexpression of GmNF-YC14 or GmPYR1 could increase yield per plant, grain plumpness, and stem base circumference, thus indicating improved adaptation of soybean plants to drought conditions. 


结论:Taken together, our findings expand the known functional scope of the NF-Y transcription factor functions and raise important questions about the integration of ABA signalling pathways in plants. Moreover, GmNF-YC14 and GmPYR1 have potential for application in the improvement of drought tolerance in soybean plants.


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 摘 要 


干旱和盐胁迫是全球范围内大豆产量的主要限制性因素。然而,由于性状的复杂性以及影响大豆产量的因素众多,提升大豆在胁迫环境下的产量十分困难。本文中,作者鉴定到了一个核因子Y C 亚基NF-YC家族转录因子GmNF-YC14,该蛋白会和GmNF-YA16以及GmNF-YB2形成一个异源三聚体,激活GmPYR1介导的脱落酸信号转导途径,从而调控大豆的胁迫响应。尤其是,作者发现通过CRISPR/Cas9技术获得的GmNF-YC14敲除突变体对于干旱的敏感性要比野生型更高。此外,田间试验显示GmNF-YC14基因或者GmPYR1基因的过表达能够增加单株的产量、籽粒饱满度和茎基周长,因此表明其对于大豆适应干旱条件的促进作用。综上,本文的研究拓宽了我们对于NF-Y转录因子功能的认知,提出了植物中有关ABA信号转导整合的重要问题。此外,GmNF-YC14GmPYR1基因在大豆植株抗旱育种改良方面具有潜在的应用前景。


 通讯作者 

** 马有志 **


个人简介:

1986年,吉林农业大学,学士;

1986-1988年,黑龙江省农科院,硕士;

1988-1994年,日本鸟取大学,硕士、博士。


研究方向植物抗逆基因工程,主要开展重要基因克隆与功能解析,小麦抗逆分子育种研究。


doi: https://doi.org/10.1111/pbi.13684


Journal: Plant Biotechnology Journal

Published onlineOctober 01, 2021



https://blog.sciencenet.cn/blog-3158122-1306771.html

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