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Current Biology:地钱MpBES1调控细胞分裂和分化

已有 706 次阅读 2021-9-26 09:10 |个人分类:每日摘要|系统分类:论文交流

The BES1/BZR1-family transcription factor MpBES1 regulates cell division and differentiation in Marchantia polymorpha

第一作者Martin A. Mecchia

第一单位西班牙巴塞罗那自治大学

第一通讯Ana I. Caño-Delgado


 Abstract 


背景回顾Brassinosteroids (BRs) play essential roles in growth and development in seed plants; disturbances in BR homeostasis lead to altered mitotic activity in meristems and organ boundaries and to changes in meristem determinacy. An intricate signaling cascade linking the perception of BRs at the plasma membrane to the regulation of master transcriptional regulators belonging to the BEH, for BES1 homologues, family has been described in great detail in model angiosperms. 


提出问题:Homologs of these transcription factors are present in streptophyte algae and in land plant lineages where BR signaling or function is absent or has not yet been characterized.


结果1-地钱BES1基因:The genome of the bryophyte Marchantia polymorpha does not encode for BR receptors but includes one close ortholog of Arabidopsis thaliana BRI1-EMS-SUPPRESSOR 1 (AtBES1) and Arabidopsis thaliana BRASSINAZOLE-RESISTANT 1 (AtBZR1), MpBES1


结果2-基因功能:Altered levels of MpBES1 severely compromised cell division and differentiation, resulting in stunted thalli that failed to differentiate adult tissues and reproductive organs.


结果3-MpBES1下游调控基因:The transcriptome of Mpbes1 knockout plants revealed a significant overlap with homologous functions controlled by AtBES1 and AtBZR1, suggesting that members of this gene family share a subset of common targets. 


结果4-拟南芥中表达MpBES1:Indeed, MpBES1 behaved as a gain-of-function substitute of AtBES1/AtBZR1 when expressed in Arabidopsis, probably because it mediates conserved functions but evades the regulatory mechanisms that native counterparts are subject to. 


结论:Our results show that this family of transcription factors plays an ancestral role in the control of cell division and differentiation in plants and that BR signaling likely co-opted this function and imposed additional regulatory checkpoints upon it.


 摘 要 


油菜素内酯BRs在种子植物的生长和发育过程中发挥着重要作用,BR内稳态的扰动会改变分生组织中的有丝分裂活性、器官边界以及改变分生组织的确定性。在被子植物中的大量研究揭示了一个复杂的信号转导级联,将质膜上对于BRs的感知与主要转录调控子BEH家族的BES1同源物的调控关联起来。这些转录因子在那些不存在BR信号转导或者还未鉴定到BR信号转导的链藻和陆地植物支系中同样存在。苔藓植物地钱的基因组中并不存在BR受体的编码基因,但是包括一个拟南芥BES1和BZR1的同源蛋白MpBES1。改变MpBES1的水平会严重阻碍细胞分裂和分化,导致地钱的菌体发育不良,使得其不能分化形成成体组织和生殖器官。Mpbes1敲除株系的转录组显示,其在地钱中能够调控的功能基因与拟南芥中AtBES1和AtBZR1调控的功能基因存在很大程度的重叠,说明该基因家族的成员共享了一小类的共同靶基因。确实,MpBES1在拟南芥中表达时,其可以作为AtBES1或者AtBZR1功能获得的一个替代,可能是因为其介导了保守的功能,但也规避了拟南芥中对应基因所受的调节机制。本文的研究结果表明了该家族的转录因子在控制植物细胞分裂与分化方面的功能很古老,并且BR信号转导很有可能了增选这一功能,并且增加了额外的调控检查点。


 通讯作者 

** Ana I. Caño-Delgado **


研究方向植物中油菜素内酯控制生长和适应不断变化的环境的进程,尤其是目前农业上遇到的最重要的问题,即干旱


doi: https://doi.org/10.1016/j.cub.2021.08.050


Journal: Current Biology

Published dateSeptember 15, 2021




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