背景回顾：Flooding and drought are serious constraints that reduce crop productivity worldwide. Previous studies identified genes conferring tolerance to both water extremes in various plants. 

提出问题：However, overlapping responses to flooding and drought at the genome‐scale remain obscure.

主要研究：Here, we defined overlapping and stress‐specific transcriptomic and hormonal responses to submergence, drought and recovery from these stresses in soybean (Glycine max). 

研究方法：We performed comparative RNA‐sequencing and hormone profiling, identifying genes, hormones and biological processes that are differentially regulated in an overlapping or stress‐specific manner. 

结果1-交叉响应：Overlapping responses included positive regulation of trehalose and sucrose metabolism and negative regulation of cellulose, tubulin, photosystem II and I, and chlorophyll biosynthesis, facilitating the economization of energy reserves under both submergence and drought. 

结果2-特异性响应：Additional energy‐consuming pathways were restricted in a stress‐specific manner. Downregulation of distinct pathways for energy saving under each stress suggests energy‐consuming processes that are relatively unnecessary for each stress adaptation are turned down. 

结果3-激素：Our newly developed transcriptomic‐ esponse analysis revealed that abscisic acid and ethylene responses were activated in common under both stresses, whereas stimulated auxin response was submergence‐specific. 

结论1：The energy‐saving strategy is the key overlapping mechanism that underpins adaptation to both submergence and drought in soybean. 

结论2：Abscisic acid and ethylene are candidate hormones that coordinate transcriptomic energy‐saving processes under both stresses.

结论3：Auxin may be a signaling component that distinguishes submergence‐specific regulation of the stress response.

 摘 要 

水淹和干旱是全球范围内导致作物产量降低的严重限制因素。先前的研究鉴定了这两种水分胁迫的相关抗性基因。但是，对于植物全基因组范围上对水淹和干旱的响应交叉还不清楚。本文中，作者鉴定了大豆对水淹、干旱、以及从这两种胁迫中恢复的交叉和胁迫特异性转录和激素响应进行了研究。作者通过比较转录组合和激素图谱，鉴定了大豆对两种胁迫的交叉和胁迫特异性响应的相关基因、激素和生物进程。交叉的响应包括了海藻糖、蔗糖代谢的正调控和纤维素、微管蛋白、光系统II和I、以及叶绿素生物合成的负调控，这促进了植株在水淹和干旱条件下能量储备的节约。额外的能量消耗途径受到胁迫特异的限制。在每种压力下，不同的能量节约途径的下调表明，对水淹和干旱两种压力适应相对不必要的能量消耗过程会被下调。作者新开发一个转录组响应分析方法，结果显示脱落酸和乙烯响应在两种胁迫下都会被激活，而生长素响应的刺激则是水淹特异的。能量节约策略是大豆适应水淹和干旱的关键交叉机制。脱落酸和乙烯是两种胁迫下协调转录组节能过程的候选激素。生长素可能是一种信号转导组分，它可以区分胁迫响应的水淹特异性调控。