背景回顾：Plants must coordinate photosynthetic metabolism with the daily environment and adapt rhythmic physiology and development to match carbon availability. Circadian clocks drive biological rhythms which adjust to environmental cues. Products of photosynthetic metabolism, including sugars and reactive oxygen species (ROS), are closely associated with the plant circadian clock, and sugars have been shown to provide metabolic feedback to the circadian oscillator. 

主要研究：Here, we report a comprehensive sugar-regulated transcriptome of Arabidopsis and identify genes associated with redox and ROS processes as a prominent feature of the transcriptional response. 

结果1-蔗糖促进O2–：We show that sucrose increases levels of superoxide (O2–), which is required for transcriptional and growth responses to sugar. 

结果2-O2–作用于蔗糖介导的昼夜节律：We identify circadian rhythms of O2–-regulated transcripts which are phased around dusk and find that O2– is required for sucrose to promote expression of TIMING OF CAB1 (TOC1) in the evening. 

结论：Our data reveal a role for O2– as a metabolic signal affecting transcriptional control of the circadian oscillator in Arabidopsis.

 摘 要 

  要

植物必须协调光合代谢和日环境，从而可以根据碳的可利用性形成生理和发育节律。生物钟根据环境信号来驱动生物节律。光合代谢的产物包括了蔗糖和活性氧物质ROS，与植物的生物钟密切相关，相关研究已经发现蔗糖为节律振荡器提供了代谢反馈。本文中，作者报道了拟南芥的蔗糖调节转录组，发现其中与氧化还原和ROS相关基因的表达是主要的转录响应。作者发现蔗糖增加了超氧阴离子O2–的含量，而这是植物对蔗糖的转录和生长响应所必需的。作者鉴定了受O2–调控的转录本的生物钟节律，发现主要阶段是在黄昏前后，并发现蔗糖促进TOC1基因在晚上的表达需要O2–。本文的研究揭示了O2–作为一个代谢信号，影响着拟南芥生物钟震荡器的转录调控。