Single-nucleus RNA and ATAC sequencing reveals the impact of chromatin accessibility on gene expression in Arabidopsis roots at the single-cell level

第一作者：Andrew Farmer

第一单位：美国国家基因组资源中心

通讯作者：Marc Libault

 Abstract 

背景回顾：Similar to other complex organisms, plants consist of diverse and specialized cell types. The gain of unique biological functions of these different cell types is the consequence of the establishment of cell-type-specific transcriptional programs.

主要研究：As a necessary step in gaining a deeper understanding of the regulatory mechanisms controlling plant gene expression, we report the use of single nucleus RNA sequencing (sNucRNA-seq) and single nucleus Assay for Transposase Accessible Chromatin sequencing (sNucATAC-seq) technologies on Arabidopsis roots.

结果1-数据可靠性：The comparison of our single nuclei transcriptomes to published protoplast transcriptomes validated the use of nuclei as biological entities to establish plant cell-type-specific transcriptomes.

结果2-新细胞类型：Furthermore, our sNucRNA-seq results uncovered the transcriptomes of additional cell subtypes not identified by single-cell RNA-seq.

结果3-sNucATAC-seq：Similar to our transcriptomic approach, the sNucATAC-seq approach led to the distribution of the Arabidopsis nuclei into distinct clusters suggesting the differential accessibility of chromatin between groups of cells according to their identity.

结果4-sNucRNA+sNucATAC：To reveal the impact of chromatin accessibility on gene transcription, we integrated sNucRNA-seq and sNucATAC-seq data and demonstrated that cell-type-specific marker genes display cell-type-specific patterns of chromatin accessibility.

结论：Our data suggest that the differential chromatin accessibility is a critical mechanism to regulate gene activity at the cell-type level.

 通讯作者 

**Marc Libault**

doi: https://doi.org/10.15252/embj.2020104559

Journal: Molecular Plant

Published date: Jan 06, 2021