Transcriptional Landscape of Rice Roots at the Single Cell Resolution

第一作者：Qing Liu

第一单位：中国农科院生物技术研究所

通讯作者：Xiaofeng Gu

 Abstract 

背景回顾：There are two main types of root systems in flowering plants, namely taproot systems of dicots and fibrous root systems found in monocots.

提出问题：Despite this fundamental split, our current knowledge of cellular and molecular mechanism driving root development is mainly based on studies of the dicot model Arabidopsis. However, the world major crops are monocots and little is known about the transcriptional programs underlying cell type specification in this clade.

主要研究：Here, we report the transcriptomes of more than 20,000 single cells derived from root tips of two agronomically important rice cultivars.

结果1-细胞类型与标记鉴定：Using combined computational and experimental analyses we were able to robustly identify most of the major cell types and define novel cell type specific marker genes for both cultivars.

结果2-水稻栽培种间的保守性：Importantly, we found divergent cell types to be associated with specific regulatory programs including phytohormone biosynthesis, signaling and response, which were well conserved between the two rice cultivars.

结果3-水稻与拟南芥间的差异性：In addition, we detected substantial differences between the cell-type transcript profiles of Arabidopsis and rice. These species-specific features emphasize the importance of analyzing tissues across diverse model species including rice.

结论：Taken together, our study provides insight into the transcriptomic landscape of major cell types of the rice root tip at single-cell resolution and opens new avenues to study cell type specification, function and evolution in plants.

 通讯作者 

doi: https://doi.org/10.1016/j.molp.2020.12.014

Journal: Molecular Plant

Published date: Dec 19, 2020