Desert truffle genomes reveal their reproductive modes and new insights into plant–fungal interaction and ectendomycorrhizal lifestyle

第一作者：José Eduardo Marqués‐Gálvez

第一单位：西班牙穆尔西亚大学

通讯作者：Asunción Morte

 Abstract 

背景回顾：Desert truffles are edible hypogeous fungi forming ectendomycorrhizal symbiosis with plants of Cistaceae family.

提出问题：Knowledge about the reproductive modes of these fungi and the molecular mechanisms driving the ectendomycorrhizal interaction is lacking.

主要研究1-基因组：Genomes of the highly appreciated edible desert truffles Terfezia claveryi Chatinand Tirmania nivea Trappe have been sequenced and compared with other Pezizomycetes.

主要研究2-转录组：Transcriptomes of T. claveryi × Helianthemum almeriense mycorrhiza from well‐watered and drought‐stressed plants, when intracellular colonizations is promoted, were investigated.

结果1-比较基因组揭示松露特异性：We have identified the fungal genes related to sexual reproduction in desert truffles and desert‐truffles‐specific genomic and secretomic features with respect to other Pezizomycetes, such as the expansion of a large set of gene families with unknown Pfam domains and a number of species or desert‐truffle‐specific small secreted proteins differentially regulated in symbiosis.

结果2-比较转录组鉴定关键基因：A core set of plant genes, including carbohydrate, lipid‐metabolism, and defence‐related genes, differentially expressed in mycorrhiza under both conditions was found.

结论：Our results highlight the singularities of desert truffles with respect to other mycorrhizal fungi while providing a first glimpse on plant and fungal determinants involved in ecto to endo symbiotic switch that occurs in desert truffle under dry conditions.

doi: https://doi.org/10.1111/nph.17044

Journal: New Phytologist

Published online: Dec 10, 2020