Messenger RNA 5' NAD+ Capping Is a Dynamic Regulatory Epitranscriptiome Mark That Is Required for Proper Response to Abscisic Acid in Arabidopsis

第一作者：Xiang Yu

第一单位：宾夕法尼亚大学

通讯作者：Brian D. Gregory

 Abstract 

研究背景：Although eukaryotic messenger RNAs (mRNAs) normally possess a 5′ end N7-methyl guanosine (m7G) cap, a non-canonical 5′ nicotinamide adenine dinucleotide (NAD+) cap can tag certain transcripts for degradation mediated by the NAD+ decapping enzyme DXO1.

提出问题：Despite this importance, whether NAD+ capping dynamically responds to specific stimuli to regulate eukaryotic transcriptomes remains unknown.

主要研究：Here, we reveal a link between NAD+ capping and tissue- and hormone response-specific mRNA stability.

结果1-DXO1突变：In the absence of DXO1 function, transcripts displaying a high proportion of NAD+capping are instead processed into RNA-dependent RNA polymerase 6-dependent small RNAs, resulting in their continued turnover likely to free the NAD+ molecules.

结果2-NAD+加帽转录本响应ABA：Additionally, the NAD+-capped transcriptome is significantly remodeled in response to the essential plant hormone abscisic acid in a mechanism that is primarily independent of DXO1.

结论：Overall, our findings reveal a previously uncharacterized and essential role of NAD+ capping in dynamically regulating transcript stability during specific physiological responses.

 通讯作者 

doi: https://doi.org/10.1016/j.devcel.2020.11.009

Journal: Developmental Cell

Published date: Dec 07, 2020