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第一作者:Dongmei Wang
第一单位:海洋生物遗传与育种教育部重点实验室
通讯作者:Yunxiang Mao
Abstract
背景-条斑紫菜: Changes in atmospheric CO2 concentration have played a central role in algal and plant adaptation and evolution. The commercially important red algal genus, Pyropia (Bangiales) appears to have responded to inorganic carbon (Ci) availability by evolving alternating heteromorphic generations that occupy distinct habitats.
背景-生活史:The leafy gametophyte inhabits the intertidal zone that undergoes frequent emersion, whereas the sporophyte conchocelis bores into mollusk shells. 主要研究:Here, we analyze a high-quality genome assembly of Pyropia yezoensis to elucidate the interplay between Ci availability and life cycle evolution. 结果:We find horizontal gene transfers from bacteria and expansion of gene families (e.g. carbonic anhydrase, anti-oxidative related genes), many of which show gametophyte-specific expression or significant up-regulation in gametophyte in response to dehydration. In conchocelis, the release of HCO3- from shell promoted by carbonic anhydrase provides a source of Ci. This hypothesis is supported by the incorporation of 13C isotope by conchocelis when co-cultured with 13C-labeled CaCO3.
摘 要
大气中二氧化碳浓度的变化在藻类和植物的适应和演化过程中扮演着非常重要的作用。红毛菜目的紫菜属具有非常重要的商业价值,其通过演化出占据不同生境的交替型世代来响应无机碳的可利用性。叶状配子体生活在潮间带环境中,经常在低潮时干出,而丝状孢子体则钻入软体动物的贝壳中。本文中,作者分析了条斑紫菜的高质量基因组,研究了无机碳可利用性与条斑紫菜生命周期演化之间的互作。作者在条斑紫菜基因组中鉴定到了来自细菌的水平转移基因,碳酸酐酶、抗氧化相关基因等基因家族发生了明显的扩张,并且其中很多基因显示出了配子体特有表达特征,亦或是在响应脱水胁迫时显著在配子体中上调表达。在条斑紫菜的丝状体中,由碳酸酐酶所促进的甲壳HCO3-释放是无机碳的一个来源。当与13C标记的CaCO3共培养时,丝状体会吸收13C同位素,证明了上文作者的推论。
通讯作者
个人简介: 2001年,中国海洋大学,博士。 研究方向: (1)藻类保护遗传学与种质保藏技术; (2)藻类基因组学与重要经济及抗逆性状分子遗传机制; (3)红藻多细胞化与有性生殖的起源与进化; (4)藻类遗传育种学与分子育种技术体系构建及良种选育; (5)藻类繁育生物学与生态栽培新技术。
doi: 10.1038/s41467-020-17689-1
Journal: Nature Communications
Published date: August 12, 2020
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