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第一作者:Panyu Yang
第一单位:北京大学
通讯作者:Haodong Chen
Abstract
背景回顾: Light and gravity are two key environmental factors that control plant growth and architecture.
提出问题: However, the molecular basis of the coordination of light and gravity signaling in plants remains obscure. 主要发现: Here, we report that two classes of transcription factors, PHYTOCHROME INTERACTING FACTORS (PIFs) and ELONGATED HYPOCOTYL5(HY5), can directly bind and activate the expression of LAZY4, a positive regulator of gravitropism in both shoots and roots in Arabidopsis. 结果-地上: In hypocotyls, light promotes degradation of PIFs to reduce LAZY4 expression, which inhibits the negative gravitropism of hypocotyls. LAZY4 overexpression can partially rescue the negative gravitropic phenotype of pifq in the dark without affecting amyloplast development. Our identification of the PIFs-LAZY4 regulatory module suggests the presence of another role for PIF proteins in gravitropism, in addition to a previous report demonstrating that PIFs positively regulate amyloplast development to promote negative gravitropism in hypocotyls. 结果-地下: In roots, light promotes accumulation of HY5 proteins to activate expression of LAZY4, which promotes positive gravitropism in roots. 结论:Together, our data indicate that light exerts opposite regulation of LAZY4 expression in shoots and roots by mediating the protein levels of PIFs and HY5, respectively, to inhibit the negative gravitropism of shoots and promote positive gravitropism of roots in Arabidopsis. 摘 要
光和重力是控制植物生长和结构的两个关键环境因子。然而,植物中协调光和重力信号转导的分子基础还亟待研究。本文中,作者发现拟南芥中光敏色素互作因子PIFs和bZIP转录因子HY5可以直接结合LAZY4基因并激活该基因的表达,而LAZY4基因同时是拟南芥地上和地下组织中向重力性的正调控因子。在下胚轴中,光促进PIFs的降解,从而降低LAZY4基因的表达,抑制下胚轴的负向重力性。LAZY4基因的过表达可以在黑暗条件下,部分拯救pifq的负向重力性表型,并且不会影响造粉质体的发育。作者鉴定到的PIFs-LAZY4调控模块说明PIF蛋白除了在之前的报道中所揭示的PIFs通过正向调控造粉体发育,从而促进下胚轴的负向重力性,还在向重力性中发挥另外的功能。在根中,光会促进HY5蛋白的积累,从而激活LAZY4基因的表达,进而促进根中的正向重力性。综上,本文的研究揭示了光通过对PIFs和HY5的蛋白水平的调节,分别对拟南芥地上和地下组织中LAZY4基因的表达进行完全相反的调控,从而抑制地上组织下胚轴的负向重力性以及促进地下组织根的正向重力性。
通讯作者
**陈浩东** 个人简介: 1999-2003年,北京大学,学士; 2006-2008年,耶鲁大学,交换生; 2003-2008年,北京大学,博士; 2008-201年,北京大学,博后; 2010年-至今,北京大学,副研究员。 研究方向: 植物中光和重力信号转导。
doi: 10.1073/pnas.2005871117
Journal: PNAS
Published date: August 04, 2020
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