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第一作者:Yu Zhong
第一单位:中国农业大学
通讯作者:Shaojiang Chen
Abstract
背景回顾:Doubled haploid technology using inducer lines carrying mutations in ZmPLA1/MTL/NLD and ZmDMP has revolutionized traditional maize breeding.
提出问题:ZmPLA1/MTL/NLD is conserved in monocots and has been used to extend the system from maize to other monocots, but no functional orthologue has been identified in dicots, while ZmDMP-like genes exist in both monocots and dicots. 主要结果一:Here, we report that loss-of-function mutations in the Arabidopsis thaliana ZmDMP-like genes AtDMP8 and AtDMP9 induce maternal haploids, with an average haploid induction rate of 2.1 ± 1.1%. 主要结果二:In addition, to facilitate haploid seed identification in dicots, we established an efficient FAST-Red fluorescent marker-based haploid identification system that enables the identification of haploid seeds with >90% accuracy. 结论:These results show that mutations in DMP genes also trigger haploid induction in dicots. The conserved expression patterns and amino acid sequences of ZmDMP-like genes in dicots suggest that DMP mutations could be used to develop in vivo haploid induction systems in dicots.
摘 要
通过带有ZmPLA1/MTL/NLD和ZmDMP基因突变诱导株系的单倍体加倍技术彻底改变了传统的玉米育种工作。ZmPLA1/MTL/NLD基因在单子叶植物中十分保守,基于该基因的单倍体加倍技术已经拓展到了其它的单子叶植物中,但是在双子叶植物中并未鉴定到同源基因。而ZmDMP类基因则存在于单子叶和双子叶植物中。本文中,作者报道了拟南芥中类ZmDMP基因AtDMP8和AtDMP9的功能缺失突变能够诱导产生母本单倍体,平均的单倍体诱导率在2.1%左右,上下波动1.1%。另外,为了促进对于双子叶植物单倍体种子的鉴定筛选,作者建立了一套高效的、基于FAST-Red荧光标记的单倍体鉴定系统,能够以超过90%的精度鉴定出单倍体种子。本文的研究结果显示了DMP基因在双子叶植物同样能够诱导单倍体的形成。双子叶植物中ZmDMP类基因的保守表达模式和氨基酸序列说明DMP突变可以被用来构建双子叶植物的体内单倍体诱导系统。
通讯作者
**陈绍江**
个人简介:
1979-1983年,河南农业大学,学士; 1985-1988年,河南农业大学,硕士; 1992-1995年,东北农业大学,博士; 1995-1997年,中国农业大学,博后。 研究方向: 玉米单倍体育种技术 高油与青贮玉米育种 玉米生殖生物学 工程化作物育种等。
doi: 10.1038/s41477-020-0658-7
Journal: Nature Plants
Published date: May 15, 2020
p.s. 植物单倍体育种相关研究链接:
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