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第一作者:Shutang Tan
第一单位:奥地利科学技术研究院
通讯作者:Hong-Wei Xue
Abstract
背景回顾:Directional intercellular transport of the phytohormone auxin mediated by PIN-FORMED (PIN) efflux carriers has essential roles in both coordinating patterning processes and integrating multiple external cues by rapidly redirecting auxin fluxes.
提出问题:PIN activity is therefore regulated by multiple internal and external cues, for which the underlying molecular mechanisms are not fully elucidated.
主要发现:Here, we demonstrate that 3′-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE1 (PDK1), which is conserved in plants and mammals, functions as a molecular hub that perceives upstream lipid signalling and modulates downstream substrate activity through phosphorylation.
具体试验:Using genetic analysis, we show that the loss-of-function Arabidopsis pdk1.1 pdk1.2 mutant exhibits a plethora of abnormalities in organogenesis and growth due to defective polar auxin transport. Further cellular and biochemical analyses reveal that PDK1 phosphorylates D6 protein kinase, a well-known upstream activator of PIN proteins.
结论:We uncover a lipid-dependent phosphorylation cascade that connects membrane-composition-based cellular signalling with plant growth and patterning by regulating morphogenetic auxin fluxes.
摘 要
由PIN蛋白介导的植物生长素在细胞间的方向性转运通过快速重定向生长素流参与植物的模式进程协调和整合多个外界信号方面发挥重要作用。因此,PIN蛋白的活性受到多个内因和外因调控,然而潜在的分子机制还没有完全研究透彻。本文中,作者的研究发现动植物中都比较保守的PDK1蛋白作为分子中心,感知上游的脂质信号并通过磷酸化调控下游底物活性。通过遗传学试验,作者发现拟南芥pdk1.1 pdk1.2功能缺失突变体由于生长素的极性运输存在缺陷,导致突变体的器官发生和生长出现各种异常。进一步的细胞学和生化分析显示PDK1磷酸化D6蛋白激酶,而该酶是PIN蛋白的上游激活子。本文的研究发现了一个依赖于脂质的磷酸化级联通过调控形态发生的生长素流从而将基于膜组分的细胞信号与植物的生长和模式建成联系起来。
通讯作者
**薛红卫**
1987-1991年,上海华东师范大学,学士;
1991-1996年,中科院上海植物生理所,博士;
1996-1998年,德国马普分子植物生理学研究所,博士后。
研究方向:主要开展植物激素作用机理和植物种子发育功能基因组学研究。
doi: 10.1038/s41477-020-0648-9
Journal: Nature Plants
Published date: May 11, 2020
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