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First author: Xie Dang; Affiliations: Fujian Agriculture and Forestry University (福建农林大学): Fuzhou, China
Corresponding author: Deshu Lin
The flowers of angiosperm species typically contain specialized conical cells. Although substantial progress has been achieved regarding the mechanisms underlying flower development, little is known about how petal cells achieve final conical shape. Here, we use 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) as a fluorescent pH indicator for analyzing the apoplastic pH of conical cells in Arabidopsis and show that normal conical cell expansion requires auxin signaling and apoplastic pH changes. By combining imaging analysis and genetic and pharmacological experiments, we demonstrate that apoplastic acidification and alkalization correlate with an increase and decrease in tip sharpening of conical cells, respectively. Initial expansion of conical cells is accompanied by decreased apoplastic pH, which is associated with increased auxin signaling. Decreased auxin levels, transport, or signaling abolishes cell wall acidification and causes reduced tip sharpening and heights of conical cells. These findings provide an insight into apoplastic pH regulation of conical cell expansion.
被子植物物种的花通常具有特化的锥形细胞。尽管关于花发育相关的研究已经取得了巨大的进展,但是关于花瓣上的锥形细胞如何获取其最终的锥形形态还不清楚。本文中,作者利用8-羟基芘-1,3,6-三磺酸三钠盐(HPTS)作为荧光pH指示剂来分析拟南芥花瓣上锥形细胞中质外pH值,发现正常的锥形细胞扩张需要生长素信号以及质外pH值的改变。通过结合呈像分析以及遗传和药理学实验,作者发现质外酸化和碱化分别与锥形细胞尖端锐化程度的增加和减少有关。圆锥细胞最初的扩张伴随着质外pH值的降低,而这与生长素信号的增强有所关联。生长素含量、转运以及信号转导水平的降低均能够使得细胞壁不再酸化,从而降低锥形细胞尖端的锐化程度和高度。本文的研究发现为植物花瓣上锥形细胞扩张的质外pH值调控提供了新的视野。
p.s. 质外体(apoplast),是指植物细胞原生质体外围由细胞壁、胞间隙和导管组成的系统。
锥形细胞,是被子植物花瓣上的一个特化结构,能够为昆虫授粉提供一个稳定的“着陆平台”。
酸生长理论,生长素通过激活细胞膜上的H+ATPase,将胞内的H+泵到胞外,使得细胞壁pH值降低、酸化,从而促进细胞壁松弛,最终导致细胞的扩张。
Highlight
Detection of apoplastic pH in conical cells using HPTS
利用HPTS检测锥形细胞中非原生质体的pH值
pH correlates with conical cell expansion
pH值与锥形细胞的扩张有关
Auxin signaling influences conical cell expansion
生长素信号会影响锥形细胞的扩张
Auxin influences pH and conical cell expansion
生长素影响pH值与锥形细胞扩张
通讯:林德书 (http://net.fafu.edu.cn/hist/15/40/c5708a136512/page.htm)
个人简介:2000-2004年,福建农林大学,学士;2004-2007年,福建农林大学,硕士;2007-2012年,中国农业大学,博士;2009-2011年,美国加州大学河滨分校,联合培养;2012-2014年, 中科院植物逆境生物学研究中心,博士后;2012-2013年,美国加州大学河滨分校,访问学者;2014年-至今,福建农林大学,教授。
研究方向:1.花瓣器官形态建成;2. 花瓣锥形细胞形态建成;3. 细胞骨架蛋白在水稻、甘蔗等农作物中的功能研究。
doi: https://doi.org/10.1016/j.celrep.2020.02.087
Journal: Cell Reports
Published date: March 17, 2020
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