Resequencing of 683 common bean genotypes identifies yield component trait associations across a north–south cline

First author: Jing Wu; Affiliations: CAAS Institute of Crop Sciences (农科院作物所): Beijing, China

Corresponding author: Shumin Wang

We conducted a large-scale genome-wide association study evaluation of 683  common bean accessions, including landraces and breeding lines, grown over 3  years and in four environments across China, ranging in latitude from 18.23° to 45.75° N, with different planting dates and abiotic or biotic stresses. A total of 505  loci were associated with yield components, of which seed size, flowering time and harvest maturity traits were stable across years and environments. Some loci aligned with candidate genes controlling these traits. Yield components were observed to have strong associations with a gene-rich region on the long arm of chromosome  1. Manipulation of seed size, through selection of seed length versus seed width and height, was deemed possible, providing a genome-based means to select for important yield components. This study shows that evaluation of large germplasm collections across north–south geographic clines is useful in the detection of marker associations that determine grain yield in pulses.

作者利用683份普通菜豆材料进行了大范围全基因组关联分析，这些材料包括了地方品种和育种系，生长超过3年，种植在中国4个不同的地点环境下，纬度分布范围在北纬18.23°到45.75°之间，种植日期不尽相同，面临的生物和非生物胁迫也有差异。作者共鉴定了505个位点与产量性状相关联，其中种子大小、开花时间、收割成熟期性状在不同年份和环境之间比较稳定。一些位点上的候选基因控制这些对应的性状。产量性状与1号染色体长臂上的基因富集区具有非常强的相关性。通过选择种子长度与种子宽度、高度之间的比例关系来控制种子大小被认为是可行的，从而提供了一种基于基因组的手段来选择重要的产量性状。本文的研究表明，对横跨南-北地理界限的大规模种质资源的评估在检测豆类作物产量决定的相关标记方面是十分有效的。

通讯：王述民 (http://icscaas.com.cn/rctd/cxtd/zwzzzysjyyj/sx_1/101642.htm)

个人简介：2001年，中国农业大学，博士。

研究方向：作物种质资源保护与利用。

doi: https://doi.org/10.1038/s41588-019-0546-0

Journal: Nature Genetics

Published date: December 23, 2019