Nuclear calcium signatures are associated with root development

First author: Nuno Leitão; Affiliations: John Innes Centre (约翰英纳斯中心): Norwich, UK

Corresponding author: Myriam Charpentier 

In plants, nuclear Ca2+ releases are essential to the establishment of nitrogen-fixing and phosphate-delivering arbuscular mycorrhizal endosymbioses. In the legume Medicago truncatula, these nuclear Ca2+ signals are generated by a complex of nuclear membrane-localised ion channels including the DOES NOT MAKE INFECTIONS 1 (DMI1) and the cyclic nucleotide-gated channels (CNGC) 15s. DMI1 and CNCG15s are conserved among land plants, suggesting roles for nuclear Ca2+ signalling that extend beyond symbioses. Here we show that nuclear Ca2+ signalling initiates in the nucleus of Arabidopsis root cells and that these signals are correlated with primary root development, including meristem development and auxin homeostasis. In addition, we demonstrate that altering genetically AtDMI1 is sufficient to modulate the nuclear Ca2+ signatures, and primary root development. This finding supports the postulate that stimulus-specific information can be encoded in the frequency and duration of a Ca2+ signal and thereby regulate cellular function.

在植物中，核钙离子的释放对于建立具有固氮和传递磷酸盐功能的丛枝菌根内共生系统至关重要。在豆科植物紫花苜蓿中，这些核钙离子信号是由一个定位于核膜的离子通道复合物产生的，该复合物包括DMI1和环核苷酸门控离子通道CNGC15s。 DMI1和CNCG15s基因在陆地植物之间是保守的，说明核钙离子信号传导除了作用于共生酶之外还具有其他功能。本文中，作者的研究显示核钙离子信号在拟南芥根细胞的核中起始，并且这些信号与主根发育相关，包括分生组织发育和生长素稳态。此外，作者还证明AtDMI1基因的遗传修饰足以调节核钙离子信号以及主根的发育。这一发现支持特定的刺激信息可以被编码为钙离子信号的频率和持续时间以调节细胞功能这一假说。

通讯：Myriam Charpentier (https://www.jic.ac.uk/people/myriam-charpentier/)

个人简介：1983-1987年，中山大学，学士；1993-1996年，西班牙马德里大学，博士；1997-1998年，西班牙马德里大学，博士后。

研究方向：1. 植物核钙信号传导；2. 豆科植物根瘤形成过程中的离子通道和信号传导；3. 陆地植物核钙信号的演变。

doi: https://doi.org/10.1038/s41467-019-12845-8

Journal: Nature Communications

Published date: October 25, 2019