Global Topological Order Emerges through Local Mechanical Control of Cell Divisions in the Arabidopsis Shoot Apical Meristem

First author: Matthew D.B. Jackson; Affiliations: University of Birmingham (伯明翰大学): Birmingham, UK

Corresponding author: George W. Bassel

The control of cell position and division act in concert to dictate multicellular organization in tissues and organs. How these processes shape global order and molecular movement across organs is an outstanding problem in biology. Using live 3D imaging and computational analyses, we extracted networks capturing cellular connectivity dynamics acrossthe Arabidopsis shoot apical meristem (SAM) and topologically analyzed the local and global properties of cellular architecture. Locally generated cell division rules lead to the emergence of global tissue-scale organization of the SAM, facilitating robust global communication. Cells that lie upon more shorter paths have an increased propensity to divide, with division plane placement acting to limit the number of shortest paths their daughter cells lie upon. Cell shape heterogeneity and global cellular organization requires KATANIN, providing a multiscale link between cell geometry, mechanical cell-cell interactions, and global tissue order.

细胞位置和分裂的控制与组织和器官中多细胞组织协同一致。然而这些进程是如何改变整体的细胞排列以及分子层面器官间的迁移是生物学上的一个比较引人注目的问题。本文利用3D成像和计算机分析，作者捕获了拟南芥茎尖分生组织的细胞连接动态网络，并且分析了细胞结构局部和整体的特性。局部产生的细胞分裂规则直接决定了SAM整体层面的组织出现，从而促进了整体的细胞交流。那些位于路径比较短的细胞更加倾向于分裂，而分裂板的位置则限制了子细胞所在最短路径的数量。细胞形状的异质性以及整体的细胞组织需要KATANIN基因提供一个细胞几何结构、细胞与细胞之间的机械互作以及整体组织排列之间多个尺度范围的连接。

通讯：George W. Bassel (https://www.birmingham.ac.uk/staff/profiles/biosciences/bassel-george.aspx)

研究方向：分子组份之间的互作是如何增加细胞行为的；细胞间的互作是如何影响器官的功能的。实验室网址：http://www.georgebassellab.com。

doi: https://doi.org/10.1016/j.cels.2018.12.009

Journal: Cell Systems

Published date: January 16, 2019