DNA demethylase ROS1 negatively regulates the imprinting of DOGL4 and seed dormancy in Arabidopsis thaliana

First author: Haifeng Zhu; Affiliations: Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences (中科院分子植物科学卓越创新中心): Shanghai, China

Corresponding author: Jian-Kang Zhu (朱健康)

Genomic imprinting (基因组印记) is a form of epigenetic regulation resulting in differential gene expression that reflects the parent of origin. In plants, imprinted gene expression predominantly occurs in the seed endosperm. Maternal-specific DNA demethylation by the DNA demethylase DME frequently underlies genomic imprinting in endosperm. Whether other more ubiquitously expressed DNA demethylases regulate imprinting is unknown. Here, we found that the DNA demethylase ROS1 regulates the imprinting of DOGL4. DOGL4 is expressed from the maternal allele in endosperm and displays preferential methylation and suppression of the paternal allele. We found that ROS1 negatively regulates imprinting by demethylating the paternal allele, preventing its hypermethylation and complete silencing. Furthermore, we found that DOGL4 negatively affects seed dormancy and response to the phytohormone abscisic acid and that ROS1 controls these processes by regulating DOGL4. Our results reveal roles for ROS1 in mitigating imprinted gene expression and regulating seed dormancy.

基因组印记是表观遗传调控的一种形式，引起能够反应其亲本起源信息的基因差异表达。在植物中，印记基因的表达主要在种子胚乳中发生。由DNA去甲基化酶DME介导的母本特异性的DNA去甲基化频繁作用于胚乳中的基因组印记。而是否存在其它更广范围表达的DNA去甲基化酶调控印记还不清楚。本文作者发现DNA去甲基化酶ROS1调控DOGL4基因的印记。DOGL4基因在胚乳中由母本等位基因表达，并且表现出优先甲基化和抑制父本等位基因。作者还发现ROS1通过去甲基化父本等位基因来负调控印记，防止父本基因的超甲基化和完全沉默。此外，作者发现DOGL4基因负调控种子休眠，响应于植物激素脱落酸，并且ROS1通过调控DOGL4基因的表达来影响这些进程。本文的研究结果揭示了ROS1在缓减印记基因表达和调控种子休眠中的作用。

通讯：朱健康（http://sourcedb.sibs.cas.cn/zw/rck/201206/t20120615_3598633.html）

个人简介：1983-1987年，北京农业大学，土壤农业化学学士；1987-1990年，加州大学河滨分校，植物学硕士；1990-1993年，普度大学，植物生理学博士；1994年，洛克菲勒大学，分子生物学博士后。

研究方向：渗透胁迫的感应和信号传导；低温胁迫的感应和信号传导；耐盐和耐氧化的机制；RNA介导的DNA甲基化；DNA去甲基化及其调控。

doi: https://doi.org/10.1073/pnas.1812847115

Journal: PNAS

First Published: 28 September, 2018

（P.S. 原文下载：链接:https://pan.baidu.com/s/1qFiSsqwlF_7JjmGMbedfhQ  密码:dmu6）