Artificial MicroRNAs Promote High-Level Production of Biomolecules Through Metabolic Engineering of Phenylpropanoid Pathway

First author: Sweda Sreekumar; Affiliations: Rajiv Gandhi Centre for Biotechnology (拉吉夫·甘地生物技术中心): Kerala, India

Corresponding author: E. V. Soniya

Plant microRNAs (miRNAs) are tiny RNAs that are crucial for the biosynthesis of secondary metabolites such as phenylpropanoids (苯丙素) because they regulate the activity of key transcription factors and enzymes. Here, we have discussed the significance of miRNAs that target the mRNAs encoding for enzymes and transcription factors in the phenylpropanoid pathway. Genes encoding the miRNAs involved in the synthesis of phenylpropanoids could be considered as potential candidates for metabolic engineering. We have analyzed the construction and potential value of artificial miRNAs (amiRNAs) that could be used for scrutinizing (仔细察看) the functional importance of miRNAs in phenylpropanoid biosynthesis and manipulating the pathway for enhancing the production of secondary metabolites.

植物microRNA，简称miRNA，是一类非常小的RNA，在诸如苯丙素等植物次生代谢物的生物合成方面发挥重要作用，其主要通过调控关键转录因子及酶的活性来发挥作用。本文，作者讨论了miRNA靶向苯丙素通路中关键酶和转录因子编码mRNA的重要性。参与苯丙素的生物合成的编码miRNA基因可能作为潜在的代谢工程候选基因。作者分析了人工miRNA（amiRNA）的构造和潜在价值，可用于未来研究miRNA在苯丙素生物合成中的功能以及通过改变通路增强植物次生代谢物产量。

通讯：E. V. Soniya (https://www.rgcb.res.in/e-v-soniya.php)

个人简介：1995年，印度喀拉拉大学，博士。

研究方向：聚酮合成酶的结构生物信息学数据库（www.type3pks.in）；黑胡椒中microRNAs在胁迫调控中的作用；植物III型聚酮合酶参与苯丙素生物合成的分子鉴定。

doi: https://doi.org/10.1080/07352689.2018.1444361

Journal: Critical Reviews in Plant Sciences

First Published date: 16 March, 2018

（P.S. 欢迎关注微信公众号：微信号Plant_Frontiers）