Title: New view of graphene oxide biosafety on water environment using an eatable fish as a model

Authors: Keyi Ma, Shupeng Zhang, Baoqing Ye,Jianyong Ouyang and Gen Hua Yue

Keyi Ma and Shupeng Zhang. These authors contributed equally to this work. 

Abstract: A comprehensive evaluation on the biosafety of graphene oxide (GO) was developed by combining 16S rRNA sequencing, gene expression detection, histology and scanning electron microscope assay on fish. GO does not affect the diversity and composition of gut microbiota, but down-regulates gene expression in fish liver, suggesting that it poses a potential risk to aquatic ecosystems.