文章：16S rRNA gene-based analysis of fecal microbiota from preterm infants with and without necrotizing enterocolitis

杂志：The ISME Journal

时间：2009

研究目的：早产儿NEC与非NEC的肠道菌群特点与差异 For this study, we hypothesized that there are differences inmolecular microbial profiles of preterm infants without and with NEC, which may be critical in the etiopathogenesis of disease.

研究策略：NEC与非NEC组各10例 A total of 21 fecal samples from 20 patients were obtained including 10 infants with NEC and10 infants without NEC as control. The study set included four sets of twins.

研究结果：

    1）richness、shannon index在NEC组显著较低，The absolute richness (the number of distinct terminal restriction fragments in each sample) of the community of NEC infants (12.8±7.3) was observed to be significantly lower than that of controls (25.2±9.8, P<0.05). The Shannon diversity index, which accounts for both abundance and evenness of the species present, as calculated for NEC infants based on presence and distribution of peaks was 1.13, which was also significantly lower than that of control infants without NEC.

   2）菌群组成，The average proportion of Proteobacteria was significantly increased and the average proportion of Firmicutes was significantly decreased compared to controls.

个人总结：

       1）数据分析的力度限于当时的环境，还有待深入开展；

       2）样品的取样，是不是可以考虑胎粪，或者在抗生素使用前；

       3）在课题设计上，可以考虑适当增加一些样本，测序和分析策略做一些优化，加上时间数据的跟踪，将这个问题再深入一些。