和其他部分一样,制作图表也有一些“规则”。首先要了解目标期刊允许多少图表,不要超过这个限制。如果限定的图表数目不足以表达你的结果,可以把有些放在“补充信息”(Supplementary Information)部分,或者在正文中叙述并加个说明“(data not shown)”。不过,只有不重要的附属性结果才能这样叙述,而那些支持你假设的结果则必须展现出来。如果你的首选期刊不足以展现你的所有重要数据,你可能需要考虑换个期刊。反之,如果期刊允许的图表数目超过你的需要,也不要加上多余或不必要的图表来凑数。每个图表必须要有明确、必要的目的。
第三,图表的标注也应该独立成文,并且无需阅读全文就能完全理解。因此,缩写要么不用,要么先定义后才用,同时应该避免使用技术术语。标注应清楚说明做了什么、观察到了什么;也应简述使用了什么统计检验,给出P值,并对所用的符号进行定义。除了其中描述方法的语句,标注和图表标题都应采用现在时。比如,应该写:“Western blot showing an increase in the levels of p53 after…”而不是“Western blotting showed that the levels of p53 increased after…”。
In the chapter on abstracts I mentioned that many readers will only read the abstract of your paper, and so the abstract needed to be self-contained, describing all of the important findings and their significance. Some readers will go further than just the abstract and look at the display items to validate the findings described in the abstract, but still not read the entire paper. Thus, like the abstract, the display items in your paper (along with their associated legends) need to be able to stand alone and be understood without the need to refer to the text of the paper.
Display items include figures and tables, which are essentially graphical representations of the results described in the text. Simply put, they are the most effective and efficient way to present your results. With good figures and tables you will be able to impart to the reader exactly what you found in your study in a relatively short period of time (that is, much faster than it would take the reader to read the entire paper). Researchers in rapidly moving fields or with limited time to keep up to date with advances in their field will appreciate the rapidity with which they can be informed of your findings; journals editors and peer reviewers will appreciate the clarity. Therefore, it is worthwhile devoting some thought and attention to developing good quality figures and tables that clearly convey your results. As with the other sections of scientific manuscripts, there are certain ‘rules’ that should be followed when generating display items. First, if you have a target journal in mind already, consider how many display items they allow and ensure that you do not exceed that limit. If you have more results to describe than can be simply shown in the allowable number of display items, some may need to be included in a Supplementary Information section, or described in the text with the statement “(data not shown)”. However, only the least important or peripheral findings should be described in this way, and all findings that support your hypothesis need to be shown. Thus, you may need to consider an alternative journal if your first choice will not allow you to present all of your important data. By contrast, if a journal allows more display items than is necessary to show your findings, do not add redundant or unnecessary display items simply because you can. All display items must have a clear and necessary purpose.
Second, the data shown in figures and tables needs to be easy to interpret. Consider how much data you wish to show in a given display item and how it can be organized to convey the important message. Therefore, rather than combining multiple parameters or treatments into a single graphic, consider splitting the data across multiple simpler graphics that can be grouped together in a single figure. Remember to clearly label any graph axes, table columns and rows, and components of diagrams if appropriate. Trendlines, scale bars and the results of any statistical tests should be also shown, where relevant, for example by using an asterisk to indicate significance, or a variety of symbols to indicate different levels of significance. With large samples, report the % change or % difference as well as absolute values. Third, the legends accompanying display items need to be able to stand alone such that the display items are entirely understandable without the need to read the entire manuscript. That means abbreviations should not be used or need to be defined, and technical terms should be avoided. It should be clear exactly what was done and what was seen. Statistical tests should be briefly described in the legends, with p values given and any symbols used defined. Legends, including their headings, should be written in the present tense with the exception of any methods described within them. For example, use “Western blot showing an increase in the levels of p53 after…” rather than “Western blotting showed that the levels of p53 increased after…”.
Finally, as mentioned in the chapter on writing the results section, there should be no redundancies between the display items and the text. Therefore, do not produce a display item to show information that can easily and briefly be stated in the text, and do not duplicate information among tables and figures, for example, by making a table to show the same information already conveyed in a figure. Do not embed figures and their legends within the text of the manuscript you plan to submit. The publisher will put your text and display items into a template ‘proof’ that will be specific to the target journal. What they usually require from authors is a separate file containing display items (occasionally these can be placed at the end of a manuscript files) and a text file that includes figure and table legends listed together at the end (usually following the references). Check the instructions for authors of your intended target journal for their specific requirements.
By preparing good quality, clear display items before writing the results section, this section will practically write itself. The display items can be grouped in a logical order that progresses your argument or progressively strengthens your hypothesis. With one subsection and one display item for each of the major findings, the subsection headings will be similar to the relevant legend headings, and the text in each subsection will provide a brief description of the findings shown in each display item, complete with the results of statistical analyses, with the reader being referred to the display items for more detail.
Examples
Tables are a great way to present large amounts of necessary data with minimal description required. The table shown above is a truncated version of a table in a paper published in The Journal of Clinical Investigation (doi:10.1172/JCI37622; reproduced with permission). The data presented clearly and economically in this table would have required a considerable amount of word space to describe in the text, but the use of a table makes the information available without the need for a wordy description. All that was required to describe this in the main text was the following statement: “Clinical characteristics of all patients and tumor samples are summarized in Table 1”. The figure below, taken from the same paper, contains many of the elements of a successful display item described in this chapter and listed in the checklist below.
Checklist 1. ‘Stand alone’ legends 2. Comply with the allowable number of display items 3. Avoid redundancy among display items or between display items and text 4. Divide data showing different effects or parameters among different panels within the same display item 5. Use scale bars, trend lines and clear labels, and show the results of statistical tests 6. Avoid or define all symbols and abbreviations 7. With large samples, show % changes/differences as well as absolute values 8. Submit figures in a separate file or at the end of the manuscript file rather than embedded in the main text 9. Check the instructions for authors for any specific requirements regarding format, size, color, number of items and any other parameters