The protective effect of 3 areca inflorescence extracts (boiling water extract, ambient temperature water
extract and ethanol extract) on hydrogen peroxide-induced oxidative damage to human serum albumin was
investigated by comparing with Trolox, the water soluble vitamin E. Protein carbonyl groups, protein
hydroperoxides,1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, hydrogen peroxide
scavenging activity and total phenolic content were examined. The results showed that boiling water extract
had the most protective effect on human serum albumin damage induced by hydrogen peroxide, comparable
to Trolox, followed by the room ambient temperature water extract and ethanol extract. The total phenolic
content of boiling water extract, ambient temperature water extract, and ethanol extract was 1.949±0.003,
1.842±0.001 and 0.983±0.011 mg gallic acid equivalent/ml extract, respectively, and six phenolic
compositions such as gallic acid, coumalic acid, epicatechin, fumalic acid, naringenin and rutin have been
found in these areca inflorescence extracts. These data suggest that areca inflorescence is a new source of
natural antioxidants.
HPLC chromatogram of standard samples and AIEs
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