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屠呦呦教授青蒿素研究国际权威期刊论文 IF27.363 H指数:357
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青蒿素的发现与中医药的礼物
The discovery of artemisinin (qinghaosu) and gifts from Chinese medicine.
Tu Y.
Qinghaosu Research Center, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China. youyoutu1930cn@yahoo.com.cn
来源:Nat Med ( P 1078-8956 E 1546-170X )
年: 2011 卷: 17 期: 10 页: 1217-20
PMID: 21989013 [Pubmed]生化与分子生物学,1区
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1.
Tu YY.
来源:Zhongguo Zhong Xi Yi Jie He Za Zhi ( P 1003-5370 E ) 年: 2011 卷: 31 期: 10 页: 1301
PMID: 22097189 [Pubmed]
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Tu Y.
Qinghaosu Research Center, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China. youyoutu1930cn@yahoo.com.cn
来源:Nat Med ( P 1078-8956 E 1546-170X ) IF:27.363H指数:357年: 2011 卷: 17 期: 10 页: 1217-20
PMID: 21989013 [Pubmed]生化与分子生物学,1区
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Zhang D. Yang L. Yang LX. Wang MY. Tu YY.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
来源:Yao Xue Xue Bao ( P 0513-4870 E ) H指数:19年: 2007 卷: 42 期: 9 页: 978-81
PMID: 18050742 [Pubmed]
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To establish an HPLC-UV-ELSD method for the determination of the content of artemisinin, arteannuin B and artemisinic acid in Herba Artemisiae Annuae. The analytical column was Nucleodur RP-C18 (250 mm x 4.6 mm, 5 microm ID). The mobile phase was acetonirile-0.1% acetic acid (50: 50) and the flow rate was 1.0 mL x min(-1) with a UV detector for artemisinin, the detection wavelength at 209 nm, and the evaporative light-scattering detector (ELSD) for arteannuin B and artemisinic acid, the drift tube temperature: 50 degrees C, the nitrogen flow rate 30 psi and the gain was 50. The resolution of artemisinin, arteannuin B and artemisinic acid was good. The linear calibration curves were obtained over the range of 0.52 - 2.6 microg for artemisinin (r = 0.999 4, n = 5), 0.022 - 4.4 microg for artemisinin B (r = 0.999 9, n = 5) and 0.203 - 8.12 microg for artemisinic acid (r = 0.999 8, n = 5), separately. The mean recoveries of the three compounds were 99.45%, 102.37% and 101.10% with RSD of 2.3%, 1.7% and 0.79%, respectively. This method is simple, rapid, accurate and suitable for the determination of the content of the three compounds in the herbs.
Li WD. Dong YJ. Tu YY. Lin ZB.
Department of Pharmacology, Health Science Center, Peking University, 38 Xue-yuan Road, Beijing 100083, PR China. lwdpharma@126.com
来源:Int Immunopharmacol ( P 1567-5769 E 1878-1705 ) IF:2.472H指数:60年: 2006 卷: 6 期: 8 页: 1243-50
PMID: 16782536 [Pubmed]免疫学,4区
The aim of this study was to investigate the mechanisms of action of Dihydroarteannuin (DHA), a semi-synthesized agent from the starting material artemisinin extracted from the Chinese Traditional Herbs Artemisia annua, on ameliorating the symptoms of lupus on BXSB mice. The concentration of TNF-alpha in the culture supernatant of the peritoneal macrophages and in the sera of BXSB mice was determined by the ELISA method. NF-kappaB protein expression and translocation were assayed by the EMSA method and laser confocal scanning microscopy method, respectively. IkappaB-alpha and NF-kappaB p65 protein expression were determined by the Western blot method. Renal tissue of the BXSB mice was prepared for assaying inhibitory activity of DHA on NF-kappaB, p65 and IkappaB-alpha protein expression in vivo. The peritoneal macrophages were prepared for analysis inhibitory effects of DHA on translocation of NF-kappaB into nuclear in vitro. We found that DHA strongly reduced the production of TNF-alpha in the culture supernatant of the peritoneal macrophages and in the sera of BXSB mice in vitro or in vivo. The results demonstrated that DHA decreased the expression of NF-kappaB subunit p65 protein and the activation of NF-kappaB in the renal tissue of BXSB mice in vivo. DHA effectively inhibited the nuclear translocation of NF-kappaB in peritoneal macrophages of BXSB mice in vitro. Furthermore, it was demonstrated that the degradation of IkappaB-alpha protein was significantly inhibited by DHA. These observations suggested that the inhibitory effects of DHA on TNF-alpha production may result from the block in the NF-kappaB signaling pathway upstream of IkappaB degradation.
Dong YJ. Li WD. Tu YY.
Department of Pharmacology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100831.
来源:Zhongguo Zhong Xi Yi Jie He Za Zhi ( P 1003-5370 E ) 年: 2003 卷: 23 期: 9 页: 676-9
PMID: 14571616 [Pubmed]
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To investigate the effect of dihydro-qinghaosu (DQHS) on auto-antibody production, TNF alpha secretion and pathologic change of lupus nephritis in BXSB mice and the possible mechanism of DQHS in treating systemic lupus erythematosus (SLE).
Chen P. Tu Y. Wang F. Li F. Yang L.
Department of Parasitology, Capital University of Medical Sciences, Beijing 100054.
来源:Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi ( P 1000-7423 E ) H指数:9年: 1998 卷: 16 期: 6 页: 421-4
PMID: 12078286 [Pubmed]
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To observe the effect of dihydro qinghaosu (DQHS) on the development of Plasmodium yoelii yoelii in Anopheles stephensi and to explore the possibility of whether DQHS has preventive effect against malaria.
Tu Y.
Research Center for Qinghao and Qinghaosu, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
来源:Chin Med J (Engl) ( P 0366-6999 E ) IF:1.053H指数:32年: 1999 卷: 112 期: 11 页: 976-7
PMID: 11721477 [Pubmed]医学：内科,4区
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Tu YY.
来源:Zhong Yao Tong Bao ( P 0254-0029 E ) 年: 1985 卷: 10 期: 9 页: 35-6, 18
PMID: 2935314 [Pubmed]
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