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韩春雨研究争议要相信权威科学家

已有 2724 次阅读 2016-9-7 10:13 |个人分类:今日热点|系统分类:观点评述

激发“韩春雨”们活力是科技创新的关键

http://news.01ny.cn/2016/guandianreyi_0907/612645.html

（作者系国家天文台副研究员、行星科学家、中国科学院青年创新促进会理事、美国天文学会卡尔·萨根奖首位华裔得主）

F1000同行评审专家

http://f1000.com/prime/sections?keywords=DNA-guided+genome+editing+using+the+Natronobacterium+gregoryi+Argonaute&hp=2&s=grd#

国际同行评议意见如下：

"DNA-guided genome editing using the Natronobacterium gregoryi Argonaute"

Feng Gao, Xiao Z Shen, Feng Jiang, Yongqiang Wu, Chunyu Han, Nat. Biotechnol. (2016)

Comments (8):

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Unregistered Submission:

( July 23rd, 2016 3:00pm UTC )

https://www.ipscell.com/2016/07/controversy-over-crispr-challenger-ngago-irreproducibility-reported/

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Gaetan Burgio:

( July 29th, 2016 11:30am UTC )

I think I have an idea why this paper is irreproducible. This simply doesn't work. I've done some experiments to try reproduce the findings in Han's paper.I've summarized here:https://medium.com/@GaetanBurgio/my-experience-with-natronobacterium-gregoryi-argonaute-ngago-3ed8909b410c#.hl7vlhia6

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Peer 5:

( August 3rd, 2016 12:47am UTC )

I bet it cannot be reproduced too. However, your experiment is inconclusive for it. Oocyte cannot recapture the cell lines. You can only say NgAgo might not work for oocyte based on your results. I prefer just wait other people conclusively demonstrating that it cannot be reproduced in the exactly same line, rather than see someone muddling the water.

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Peer 3:

( July 29th, 2016 7:44pm UTC )

Did't work in Yeast either. I will test again with codon optimized NgAgo for Yeast. Will post the results soon

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Unregistered Submission:

( August 4th, 2016 2:14am UTC )

I think there's one thing might be a problem here. In your Experience story you said you injected the mouse zygote with the NLS-NgAgo plasmid from addgene with the guide DNA. However, the NgAgo in that plasmid is driven by a CMV promoter that might be silenced by early embryo cells. So it might worth to check whether the protein was actually expressed in the zygotes. But here come another annoying thing of the plasmid Han deposits to addgene, it doesn't have a tag for easy imumunodetection. I think somebody should fuse a tag to NgAgo and properly check whether it is expressed at all in their systems.

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Gaetan Burgio:

( August 8th, 2016 10:01pm UTC )

Thanks very much for your interesting comment. Yes, there is always a possibility the transcription might be silenced by early embryonic cells, although I have an indirect evidence the protein is there. I am still conducting experiments on NgAgo to check confirm the presence on NgAgo at early embryonic stage. I fully agree with you, a tagged protein (flag, his or whatever) would have been extremely useful to check the expression.

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Unregistered Submission:

( August 9th, 2016 10:33am UTC )

Nature have their take on it here:
http://www.nature.com/news/replications-ridicule-and-a-recluse-the-controversy-over-ngago-gene-editing-intensifies-1.20387

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Unregistered Submission:

( August 9th, 2016 2:40pm UTC )

We are currently trying it in the worm, with a codon optimized version.
I let you know if we have anything conclusive...

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