Evaluationof sulfane sulfur bioeffects via a mitochondria-targeting selenium-containingnear-infrared fluorescent probe

Min Gao,^ab【高敏】Rui Wang,^a【王锐】 Fabiao Yu,^a*【于法标】 Lingxin Chen^a*【陈令新】

^a Key Laboratory of CoastalEnvironmental Processes and Ecological Remediation, Research Centre for CoastalEnvironmental Engineering and Technology, Yantai Institute of Coastal ZoneResearch, Chinese Academy of Sciences, Yantai 264003, China.

^b University of Chinese Academy ofSciences, Beijing 100049, China.

Abstract

As a crucial member in antioxidant regulatory systems, sulfane sulfur plays essential roles in cytoprotective mechanisms by directly eliminating ROS and altering ROS-mediated redox signaling. Despite the rising interests in sulfane sulfur, there only a few bio-compatible methods are available for its direct detection. Moreover, most of the existing methods cannot meet the requirements of real-time detection due to the reactive and labile chemical properties of sulfane sulfur. Therefore, we strive to clarify the mutual relationship between mitochondria sulfane sulfur and ROS under hypoxia stress. Herein, we report a near-infrared fluorescent probe Mito-SeH for the selective imaging of mitochondrial sulfane sulfur in cells and in vivo under hypoxia stress. Mito-SeH includes three moieties: a selenol group (SeH) as the stronger sulfur-acceptor; a near-infrared azo-BODIPY fluorophore as the fluorescent modulator; a lipophilic alkyltriphenylphosphonium cation as the mitochondrial delivery. Mito-SeH exhibits excellent selectivity and sensitivity towards the detection of mitochondria sulfane sulfur. The hypoxia response behavior of Mito-SeH is evaluated in monolayer cell and three-dimensional multicellular spheroid to clarify the relationship between sulfane sulfur and hypoxia. We confirm that sulfane sulfur protection mechanism against hypoxia is to inhibition of caspase-dependent apoptosis through directly scavenging ROS pathway. The probe is also applied to measurement of sulfane sulfur in ex vivo-dissected organs of hypoxic mouse model, as well as the probe is successfully used for real-time monitoring the changes of sulfane sulfur and ROS in acute ischemia mice model. We suggest that sulfane sulfur may be a novel therapeutic agent for hypoxia-induced injury.

Biomaterials Volume 160, April 2018, Pages 1-14.

http://www.sciencedirect.com/science/article/pii/S0142961218300176