Qiu J, Pan X, Visualization of protein RecR in Escherichia coli by fluorescent labelling, Prog Nat Sci (2009),
doi:10.1016/j.pnsc.2009.07.003

Visualization of protein RecR in Escherichia coli by fluorescent labelling
Jiefang Qiu, Xuefeng Pan *
 School of Life Science, Beijing Institute of Technology, Beijing 100081, China
 Received 10 June 2009; received in revised form 3 July 2009; accepted 8 July 2009

 Abstract
 RecR protein, a functional equivalent of Rad52 in eukaryotes, plays a critical role in the RecF pathway of homologous recombination  in Escherichia coli. By constructing and expressing recR-yfp hybrid gene, the distribution of RecR-YFP fusion protein was visualized in E. coli by fluorescent microscopy. Our results showed that RecR proteins can be localized predominantly in the nucleoid region of E. coli.
By measuring the UV resistance of a recR mutant carrying recR-yfp gene in the plasmid, the expressed RecR-YFP was found to be func tional in improving the UV resistance of the recR deficiency strain.

2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in  China Press. All rights reserved.