今年Eric Betzig诺贝尔化学奖折冠，不久前science上又展现其新作品：Lattice light-sheet microscopy。

摘要：虽然荧光显微镜为活样本的生理学成像提供了视窗，很多生理过程或因太细微，或因发生的太快而无法通过现有工具捕获、展现。我们利用二维的光学晶格制作了超薄的光片，借助它可以在压秒级的时间间隔中、低达甚至超过衍射极限下，实现大体量三维立体动态成像。我们使用这一工具系统的在时空维度生成了多种事件的发生过程，包括3D培养基中干细胞团内转录因子的单分子扩散，处在有丝分裂中微管的稳定性动态变化，免疫学突触生成，中性粒细胞的运动，以及秀丽隐杆线虫和果蝇的胚胎发生等，这些结果让生物界管窥了生命系统之繁富与华美。

链接：http://www.sciencemag.org/content/346/6208/1257998.full

文题：Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

摘要：Although fluorescence microscopy provides a crucial window into the physiology of living specimens, many biological processes are too fragile, are too small, or occur too rapidly to see clearly with existing tools. We crafted ultrathin light sheets from two-dimensional optical lattices that allowed us to image three-dimensional (3D) dynamics for hundreds of volumes, often at subsecond intervals, at the diffraction limit and beyond. We applied this to systems spanning four orders of magnitude in space and time, including the diffusion of single transcription factor molecules in stem cell spheroids, the dynamic instability of mitotic microtubules, the immunological synapse, neutrophil motility in a 3D matrix, and embryogenesis in Caenorhabditis elegans and Drosophila melanogaster. The results provide a visceral reminder of the beauty and the complexity of living systems.

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