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Jun Yi , Li Jin , Jing Chen , Bing Feng , Zhenyue He , Longbang Chen, and Haizhu Song
Department of Cardiothoracic Surgery, Jinling Hospital, Nanjing 210002, China, and
Acta Biochim Biophys Sin 2017, 49: 159–169; doi: 10.1093/abbs/gmw131
Esophageal squamous cell carcinoma (ESCC) is the most common histological type in China. MicroRNAs are endogenously expressed in mammals and play a significant role in tumor invasion and metastasis by targeting potential downstream genes. In the present study, microarray analysis showed that miR-375 expression was distinctly downregulated in ESCC compared with that in normal esophageal epithelium tissues. Then, luciferase reporter assay showed that SHOX2 was the direct downstream target of miR-375 and this interaction was confirmed by the rescue experiments. Quantitative polymerase chain reaction results also showed that SHOX2 expression was upregulated in ESCC cells and tissues. Further analysis showed that SHOX2 induced proliferation, invasion, and metastasis of ESCC both in vivo and in vitro. Moreover, the interaction between miR-375 and SHOX2 affected the epithelial-to-mesenchymal transition. We conclude that miR-375 may suppress invasion and metastasis of ESCC by directly targeting SHOX2. The miR-375/SHOX2 axis may be a novel therapeutic target for ESCC.
MiR-375 inhibited proliferation, invasion and metastasis in ESCC cells via targeting SHOX2
阅读全文: http://www.abbs.org.cn/arts.asp?id=4118
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