Ginsenoside Rh2 inhibits proliferation and induces apoptosis in human leukemia cells via TNF-α signaling pathway

Jingjia Huang,  Kunjian Peng,  Linghao Wang,  Bin Wen,  Lin Zhou,  Tiao Luo,  Min Su,  Jijia Li and  Zhiyong Luo

Molecular Biology Research Center, State Key Laboratory of Medical Genetics, School of Life Sciences, Xiangya School of Medicine, Central South University, Changsha 410008, China

Acta Biochim Biophys Sin 2016, 48: 750–755; doi: 10.1093/abbs/gmw049

Ginsenoside Rh2, a triterpene saponin extracted from Panax ginseng, exhibits pharmacological activity against multiple cancers. However, the anticancer mechanism of ginsenoside Rh2 is unclear. In this study, we found that ginsenoside Rh2 effectively inhibits growth and induces apoptosis of HL-60 cells. Using microarray technology, we found that tumor necrosis factor-α (TNF-α) is clearly up-regulated. Furthermore, anti-TNF-α antibody relieved the Rh2-induced HL-60 cell apoptosis via suppression of caspase-8, caspase-9, and caspase-3 activation. In addition, TNF-α up-regulation was also observed in other Rh2-treated cancer cell lines. These results demonstrate that TNF-α plays a key role in ginsenoside Rh2-induced cell apoptosis.

The effects of TNF-α neutralizing antibody on Rh2-induced apoptosis in HL-60 cells

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