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每日翻译20190727

已有 415 次阅读 2019-7-27 16:47 |个人分类:翻译作品|系统分类:科研笔记| DNA条形码, 采样程序

#编者信息

熊荣川

明湖实验室

xiongrongchuan@126.com

http://blog.sciencenet.cn/u/Bearjazz

Collecting Information and DNA Procedures

Specimens were sampled at the edges of   temporal salt lakes and salt flats in the Provinces of Western Australia,   South Australia, and the Northern Territory during two expeditions in 2001   and 2003. Several of these sites were surveyed for cicindelids for the first   time, and collections produced many apparently undescribed species and   variants (Fig. 1). Specimens were placed directly into absolute ethanol after   cursory identification in the field. Up to six individuals (more in a few   cases) from each locality were sequenced, plus an additional set of up to six   individuals if obvious morphologically different forms were recognized during   field work at a given site or where individuals were obtained in different   years or at multiple adjacent sites on the larger lakes (Supplementary Table   S1; http://systematicbiology.org). This sampling regime was thought to be a   good reflection of the total diversity encountered at the various collecting   sites capturing species-level groups and their internal variation, given the   absence of a thorough taxonomic treatment, while keeping the sequencing   effort to a minimum. In total, 468 specimens assigned to 108 local sets of   individuals (65 sites, plus morphologically distinct forms and samples from   repeat visits of a site) were included in the analysis.

采集信息和DNA程序

标本主要是在2001年和2003年的两次考察中(野外出差、野外采集、野外考察),在西澳大利亚省、南澳大利亚省和北部地区的临时盐湖和盐滩边缘采集到的。其中有几个地方是对虎甲类进行的第一次调查,收集的标本中有许多明显未被描述的物种和变种(图1)。在野外粗略鉴定后,标本直接放入无水乙醇中。对每个地区的多达6个个体(在少数情况下更多)进行测序,如果在特定地点的野外工作中或在不同年份或在大型湖泊上多个相邻地点获得个体时发现不同个体明显形态不同,则另加一组(多达6个个体)(见附表S1http://systemcbiologiy.org)。考虑到缺乏彻底的分类处理,同时将测序工作保持在最低工作量,这一抽样方案能捕获各个采集点物种水平群体及其内部变异,对该地区总体物种多样性具有良好的反映。总共对隶属108个群体的468个样本,(依据65个采集点,加上形态上的不同形式和重复考察的位点样本)进行了分析。

Pons J, Barraclough T G, Gomez-Zurita J, et   al. Sequence-based species delimitation for the DNA taxonomy of undescribed   insects[J]. Systematic biology, 2006, 55(4): 595-609.




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