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每日翻译20190627

已有 366 次阅读 2019-6-27 07:02 |个人分类:翻译作品|系统分类:科研笔记| DNA条形码, 建库, 数据库, 应用

#编者信息

熊荣川

明湖实验室

xiongrongchuan@126.com

http://blog.sciencenet.cn/u/Bearjazz

One key step in the DNA barcoding process   is the establishment of a reliable and comprehensive database (reference   library) that includes both high-quality DNA barcode sequences and accurately   identified voucher specimens (Taberlet, Coissac, Pompanon, Brochmann &   Willerslev, 2012). Once established, such databases can become a valuable   toolkit for molecular ecologists to identify plant material such as pollen,   seeds, seedlings, roots, and even bark fragments (Liu et al., 2018). DNA   barcoding is now becoming essential for a broad range of ecological   applications, such as biodiversity assessments (Lahaye et al., 2008; Yoccoz   et al., 2012), establishing pollination networks (Bell et al., 2016; Vamosi,   Gong, Adamowicz & Packer, 2017), diet analysis (Kartzinel et al., 2015;   Valentini, Pompanon & Taberlet, 2009), and determining the mechanisms   underlying community assembly (Kress et al., 2009, 2010; Pei et al., 2011).

 

DNA条形码过程中的一个关键步骤是建立一个可靠和全面的数据库(参考库),其中包括高质量的DNA条形码序列和准确鉴定的凭证标本(Taberlet, Coissac, Pompanon, Brochmann & Willerslev, 2012)。一旦建立,这些数据库就可以成为分子生态学家识别植物材料(如花粉、种子、幼苗、根甚至树皮碎片)的宝贵工具(Liu et al., 2018)。DNA条形码现在已成为生态研究多个方面的基础,如生物多样性评估(Lahaye et al., 2008; Yoccoz et al., 2012)、授粉网络建立(Bell et al., 2016; Vamosi, Gong, Adamowicz & Packer, 2017)、食性分析(Kartzinel et al., 2015; Valentini, Pompanon & Taberlet, 2009)和居群构成解析(Kress et al., 2009, 2010; Pei et al., 2011)。

Shao-Lin T , Ya-Huang L , Hollingsworth P   M , et al. DNA barcoding herbaceous and woody plant species at a subalpine   forest dynamics plot in Southwest China[J]. Ecology and Evolution, 2018.




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