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氢气治疗慢性氧中毒

已有 5649 次阅读 2010-4-24 19:48 |个人分类:氢气生理盐水|系统分类:论文交流|关键词:氢气| 氢气

    到目前为止,氢气抗氧化的研究几乎包括所有与氧化损伤有关的疾病:缺血再灌注、炎症、药物毒性、创伤、放射损伤、氧中毒。将来的研究内容需要在深度和机制上更进一步,在这样简单更换模型验证治疗效果的研究发表论文将逐渐困难。

Undersea Hyperb Med. 2010 May-Jun;37(3):185-92.全文下载

Saturated hydrogen saline protects the lung against oxygen toxicity.

Zheng J, Liu K, Kang Z, Cai J, Liu W, Xu W, Li R, Tao H, Zhang JH, Sun X.

Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China.

Abstract

Exposure to high oxygen concentrations leads to acute lung injury, including lung tissue and alveolar edema formation, congestion, intra-alveolar hemorrhage, as well as endothelial and epithelial cell apoptosis or necrosis. Several studies have reported that molecular hydrogen is an efficient antioxidant by gaseous rapid diffusion into tissues and cells. Moreover, consumption of water with dissolved molecular hydrogen to a saturated level (hydrogen water) prevents stress-induced cognitive decline in mice and superoxide formation in mice. The purpose of the present study was to investigate the effect of saturated hydrogen saline on pulmonary injury-induced exposure to >98% oxygen at 2.5 ATA for five hours. Adult male Sprague-Dawley (SD) rats were randomly divided into three groups: control group, saline group and saturated hydrogen saline group. Hematoxylin and eosin (H&E) staining were used to examine histological changes. The lung wet to dry (W/D) weight ratio was calculated. The concentration of protein and total cell counts in bronchoalveolar lavage fluid (BALF) were measured. Lactate dehydrogenase (LDH) in serum and BALF were measured by spectrophotometer. The light microscope findings showed that saturated hydrogen saline reduced the impairment when compared with the saline group: Saturated hydrogen saline decreased lung edema, reduced LDH activity in BALF and serum, and decreased total cells and protein concentration in BALF. These results demonstrated that saturated hydrogen saline alleviated hyperoxia-induced pulmonary injury, which was partly responsible for the inhibition of oxidative damage.

PMID: 20568549 [PubMed - in process]

 Saturated hydrogen saline protects lung against oxygen toxicity

 

Abstract

Exposure to high oxygen concentration leads to acute lung injury, including lung tissue and alveolar edema formation, congestion, intra-alveolar hemorrhage, as well as  endothelial and epithelial cell apoptosis or necrosis. Several studies have reported that molecular hydrogen is an efficient antioxidant by gaseous rapid diffusion into tissues and cells. Moreover, consumption of water with dissolved molecular hydrogen to a saturated level (hydrogen water) prevents stress-induced cognitive decline in mice, and the superoxide formation in mice. The purpose of the present study was to investigate the effect of saturated hydrogen saline on pulmonary injury induced exposure to >98% oxygen at 2.5 ATA for 5 hours. Adult male Sprague-Dawley (SD) rats were randomly divided into three groupscontrol group, saline group, and saturated hydrogen saline group. Hematoxylin and eosin (H&E) staining were used to examine histological changes. The lung wet to dry weight ratio was calculated. The concentration of protein and total cell counts in bronchoalveolar lavage fluidBALFwere measured. Lactate dehydrogenase (LDH) in serum and BALF were measured by spectrophotometer. The light microscope findings showed that  saturated hydrogen saline reduced the impairment when compared with saline group; saturated hydrogen saline decreased lung edema, reduced LDH activity in BALF and serum, and decreased total cells and protein concentration in BALF. These results demonstrated that saturated hydrogen saline alleviated hyperoxia-induced pulmonary injury, which was partly responsible for the inhibition of oxidative damage .

本文将在潜水高气压医学领域国际著名杂志6月“水下高气压医学”发表。

“氧中毒”一般发生在长期吸氧的病人中。尽管适当吸氧能提高人体细胞新陈代谢能力、增强人体免疫力,但长期吸入高浓度氧气也会发生肺泡表面活性物质减少,引发肺泡内渗液,出现肺水肿,出现头昏、面色苍白、心跳加快等诸多问题。 
    更为严重的是,氧中毒当时不容易被觉察,往往在2-3天后才会发生临床症状,此时再进行抢救往往容易贻误时间。
氧中毒一般分为三种:
1.  肺型氧中毒 
    开始为鼻粘膜充血,有发痒感觉。即可出现口干、咽痛、咳嗽、胸骨后不适;发生频繁咳嗽、吸气时胸骨后灼痛;胸骨后剧痛、难以控制的咳嗽,肺活量已出现下降,危及生命。由于在临床呼吸氧气就可以发生这类情况,一直是非常受关注的问题。
2. 脑型氧中毒 
    最初出现额、眼、鼻、口唇及面颊肌肉的纤维性颤动,也可累及手的小肌肉;面色苍白、有异味感。继而可有恶心、呕吐、眩晕、汗、流涎、上腹部紧张;也可出现视力丧失、视野缩小、幻视、幻听;还会有心动过缓、心悸、气哽、指 (趾)端发麻、情绪反常(忧虑、抑郁、烦躁或欣悦)。接着出现极度疲劳、嗜睡、呼吸困难等。这种情况很少发生,一般在呼吸氧分压超过3个大气压才有可能发生。只有在潜水过程中才有可能发生这样的情况。部分敏感人2个大气压也有可能发生。 
3. 眼型氧中毒:
    主要表现为视网膜萎缩。 早产婴儿在恒温箱内吸氧时间过长,视网膜有广泛的血管阻塞、成纤维组织浸润、晶体后纤维增生,可因而致盲。 
在自由基生物学的早期就有人注意到,氧的毒性与体内产生大量活性氧有关,氧气的毒性效应一直是潜水医学关注的问题。我们开始研究氢气抗氧化作用的早期就进行了这方面的研究。但可惜的是,在急性氧中毒动物实验中,我们没有发现任何作用。在慢性中毒模型中,尽管有一定作用,但不是非常理想,本文是对这个研究的初步报道。我们将继续深入研究。


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