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高压氧预适应保护脊髓缺血损伤

已有 5884 次阅读 2009-2-9 12:08 |个人分类:高压氧|系统分类:科研笔记| 论文, 高压氧, 预适应, 预适应, 预适应

这个文章的投稿过程比较复杂,而且这个杂志收费比较厉害.但比较认真.

我们从NO途径来探讨预适应效果,从机制上有了一点深入.

 

 J Neurotrauma. 2009 Jan 18. [Epub ahead of print]

Hyperbaric Oxygen Preconditioning Attenuates Early Apoptosis after Spinal Cord Ischemia in Rats.

1 Department of Anesthesiology, Changhai Hospital Affiliated to Second Military Medical University , Shanghai, P.R. China ., 2 Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University , Shanghai, P.R. China ., 3 Department of Physiology and Pharmacology, Loma Linda University School of Medicine , Loma Linda, California.

Abstract This study tested the hypothesis that spinal cord ischemic tolerance induced by hyperbaric oxygen preconditioning (HBO-PC) is mediated by inhibition of early apoptosis. Male Sprague-Dawley rats were preconditioned with consecutive 4 cycles of 1-h HBO exposures (2.5 atmospheres absolute [ATA], 100% O(2)) at a 12-h interval. At 24 h after the last HBO pretreatment, rats underwent 9 min of spinal cord ischemia induced by occlusion of the descending thoracic aorta in combination with systemic hypotension (40 mmHg). Spinal cord ischemia produced marked neuronal death and neurological dysfunction in animals. HBO-PC enhanced activities of Mn-superoxide dismutase (Mn-SOD) and catalase, as well as the expression of Bcl-2 in the mitochondria in the normal spinal cord at 24 h after the last pretreatment (before spinal cord ischemia), and retained higher levels throughout the early reperfusion in the ischemic spinal cord. In parallel, superoxide and hydrogen peroxide levels in mitochondria were decreased, cytochrome c release into the cytosol was reduced at 1 h after reperfusion, and activation of caspase-3 and -9 was subsequently attenuated. HBO-PC improved neurobehavioral scores and reduced neuronal apoptosis in the anterior, intermediate, and dorsal gray matter of lumbar segment at 24 h after spinal cord ischemia. HBO-PC increased nitric oxide (NO) production. L-nitroarginine-methyl-ester (L-NAME; 10 mg/kg), a nonselective NO synthase (NOS) inhibitor, applied before each HBO-PC protocol abolished these beneficial effects of HBO-PC. We conclude that HBO-PC reduced spinal cord ischemia-reperfusion injury by increasing Mn-SOD, catalase, and Bcl-2, and by suppressing mitochondrial apoptosis pathway. NO may be involved in this neuroprotection.

PMID: 19196076 [PubMed - as supplied by publisher]

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