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146种常用培养基配方

已有 10283 次阅读 2007-10-11 13:37 |个人分类:生命科学—实验技术

 

THE COMPOSITION OF MEDIA
培养基及成分
1. Acetobacter Medium (醋酸菌培养基)
Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g
CaCO3 20g Agar (琼脂) 15g
Distilled water (蒸馏水) 1000ml Adjust (调) pH to 6.8
适用范围:恶臭醋酸杆菌混浊变种

2. Nutrient Agar (营养肉汁琼脂)
Pepton (蛋白胨) 5g Beef extract (牛肉膏) 30g
NaCl 5g Agar (琼脂) 15g
Distilled water (蒸馏水) Adjust (调) pH to 7.0-7.2
[Note]:When cultivation of Bacillus,5mg of to MnSO4.H2O may be added .
It is favorable to promote spore formation .
适用范围:产气气杆菌、粪产碱杆菌、蜡状芽孢杆菌、蜡状芽孢杆菌蕈状变种、地衣形芽孢杆菌、巨大芽孢杆菌、多粘芽孢杆菌、尘埃芽孢杆菌、短小芽孢杆菌、嗜热脂肪芽孢杆菌、枯草芽孢杆菌、枯草芽孢杆菌深黑变种、苏云金芽孢杆菌、苏云金芽孢杆菌蜡螟亚种(青虫菌)、苏云金芽孢杆菌戈尔斯德变种、苏云金芽孢杆菌猝倒亚种、产氨短杆菌、黄色短杆菌、谷氨酸棒状杆菌、北京棒杆菌、大肠埃希氏菌(大肠杆菌)、铜绿假单胞菌(绿脓杆菌)、凸形假单胞杆菌、荧光假单胞菌、弯曲假单胞菌、恶臭假单胞菌、假单胞杆菌、藤黄八叠球菌、亚黄八叠球菌、尿素八叠球菌、金黄色葡萄球菌、运动发酵单孢菌

3. Azotobacter Medium (固氮菌培养基)
KH2PO4 0.2g K2HPO4 0.8g
MgSO4.7H2O 0.2g CaSO4.2H2O  0.1g
Na2MoO4.2H2O Trace(微量) Yeast axtract(酵母膏) 0.5g
Mannitol(甘露醇) 20g FeCl3 Tract(微量)
Distilled water (蒸馏水) 1000ml Agar (琼脂) 15g
Adjust (调) pH to 7.2
适用范围:固氮菌、胶质芽孢杆菌

4. Corn Meal Medium (玉米粉培养基)
Maize flour (玉米粉) 5g Peptone (蛋白胨) 0.1g
Glucose (葡萄糖) 1g Tap water (自来水) 1000ml
[Note]:Boil the mixture in autoclave at 121℃ for 1 hr. distribute the medium into 18ⅹ18 mm tubes , each contains 10 ml of the liquid , then autoclave at 121℃ for 1 hr . again (15磅蒸煮1小时,分装入18ⅹ18毫米试管,每管深度达6厘米。15磅再次灭菌15小时。)

5. Lactic-bacteria Medium I (乳酸菌培养基 I )
Yeast extract (酵母膏) 7.5g Peptone (蛋白胨) 7.5g
Glucose (葡萄糖) 10g KH2PO4 2g
Tomato juice (西红柿汁) 100ml Tween (吐温) 80 0.5ml
Distilled water (蒸馏水) 900ml pH 7.0
适用范围:植物乳杆菌(胚芽乳杆菌)、嗜热乳酸链球菌

6. Lactic-bacteria Midium Ⅱ (乳酸菌培养基 Ⅱ)
Lacto-casein peptone (乳酪蛋白胨) 10g Beef extract (蛋白胨) 10g
Yeast extract (酵母膏) 5g Glucose (葡萄糖) 5g
Tween (吐温) 80 1g K2HPO4 2g
Na-acetate (醋酸钠) 5g Diamine citrate (柠檬酸二胺) 2g
MgSO4.7H2O 0.2g MnSO4.H2O 0.05g
Distilled water (蒸馏水) 1000m pH 6.5-6.8
适用范围:植物乳杆菌(胚芽乳杆菌)

7. Peotone Glucose Yeast extract Medium PGY (蛋白胨、酵母膏、葡萄糖培养基)
Peptone(蛋白胨) 10g Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 1g Distilled water (蒸馏水) 1L

8. Glycerol Agar (甘油琼脂)
Peptone (蛋白胨) 5g Beef extract (酵母膏) 3g
Glycerol (甘油) 20g Top water (自来水) 1000ml
Agar (琼脂) 15g pH 7.0-7.2

9. Rhizobium medium (根瘤菌培养基)AS 9
Yeast eztract (酵母膏) 1g Soil eztract (土壤浸提液) 200ml
Mannitol (甘露醇) 10g Agar (琼脂) 15g
Distilled water (蒸馏水) 800ml pH 7.2
[Note]:Soil extract:Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients and distributed. (土壤浸提液的制法:取土壤50克,加水200毫升,15磅蒸煮1小时,经滤纸过滤后加水补足到200毫升。)
适用范围:大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、
大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌

10. Mannitol Agar (甘露醇琼脂)
Yeast extract (酵母膏) 5g Peptone (蛋白胨) 3g
Mannitol (甘露醇) 25g Agar (琼脂) 15g
Distilled water (蒸馏水) 1000ml

11. Glucose Asparagine (葡萄糖、天门冬素琼脂)
Glucose (葡萄糖) 10g Asparagine (天门冬素) 0.5g
K2HPO4 0.5g Water (水) 1000ml
pH 7.2-7.4
适用范围:刺孢小单孢菌绛红变种、紫色小单孢菌(绛红小单孢菌)

12. Gause′s Synthetic Agar (高氏合成一号琼脂)
KNO3 1g Soluble starch(可溶性淀粉) 20g
K2HPO4 0.5g MgSO4.7H2O 0.5g
NaCl 0.5g FeSO4 0.01g
Agar (琼脂) 20g water (水) 1000ml
pH 7.2-7.4
适用范围:刺孢小单孢菌绛红变种、紫色小单孢菌(绛红小单孢菌)、白黄链霉菌、白色链霉菌、抗生链霉菌、双重轮丝链霉菌、产色链霉菌、烬灰链霉菌、天蓝色链霉菌、灭蚊链霉菌、红霉素链霉菌、青色链霉菌、球孢链霉菌、浅灰链霉菌、灰色链霉菌、吸水链霉菌、淡紫灰链霉菌、黄色长孢链霉菌、藤黄色链霉菌、细黄链霉菌、黑化链霉菌、玫瑰色链霉菌、华美链霉菌、嗜热链霉菌、委内瑞拉链霉菌、紫色直丝链霉菌、紫色链霉菌、绿色链霉菌

13. Wort Agar (麦芽汁琼脂)
Dilute the world (without hop) to 12 Brix. Add 15g agar into 1000ml of the diluted word..Melt the agar by heating, then distribute the medium into tubes. Autoclave at 110 for 30 minutes. (将发酵啤酒的原料(未加酒花),稀释至12柏林,加琼脂15克,溶化后分装。15磅灭菌30分钟。)
适用范围:克鲁斯假丝酵母、郎比可假丝酵母、解脂假丝酵母、马其顿假丝酵母、拟热带假丝酵母、粗壮假丝酵母、皱褶假丝酵母、热带假丝酵母、产朊假丝酵母、阿舒假囊酵母、白地霉、果香地霉、地霉属、异常汉逊酵母、异常汉逊酵母变种、阿拉伯糖醇汉逊酵母、施氏汉逊酵母、菅囊酵母、伯顿毕赤酵母、膜醭毕赤酵母、粘红酵母、小红酵母、胶红酵母、深红酵母、卡尔斯伯酵母、酿酒酵母、椰园酿酒酵母、发酵性酵母、洛格酵母、罗斯酵母、鲁氏酵母多形鲁氏酵母、威尔酵母、酵母、路德类酵母、栗酒裂殖酵母、掷抱酵母、贝雷丝孢酵母、皮状丝孢酵母、糙孢曲霉、无壳曲霉、鲜橙曲霉、红曲霉、紫红曲霉、红色红曲霉、红曲霉菌

14. Potato Dextrose Agar PDA (马铃薯、葡萄糖琼脂)
Potato extract (马铃薯汁) 1000ml Dextrose(glucose) (葡萄糖) 20g
Agar (琼脂) 20g
[Note]: Potato extract: Slice potatoes thin. Add 1000ml of water, immediately to prevent Oxidtion of juice and boil until soft. (approximately 30min.). Filter through cotton-cloth. Autoclave at 115℃ for 20 minutes.(注:取去皮马铃薯200克,切成小块,加水1000毫升煮沸30分钟,滤去马铃薯块,将滤液补足至1000毫升,加葡萄糖20克,琼脂15克,溶化后分装,15磅灭菌30分钟。)
适用范围:酵米面假单胞菌(酵米面黄杆菌)、白色链霉菌、烬灰链霉菌、青色链霉
菌、球孢链霉菌、灰色链霉菌、龟裂链霉菌、伞枝梨头霉、雅致放射毛霉、棒曲霉、
米曲霉、出芽短梗霉、白僵霉、灰葡萄孢、顶头孢霉、巴西毛壳、长刺毛壳、橄榄包
毛壳、反曲毛壳、琥珀毛壳、圆酵毛壳、束状刺盘孢、新月弯孢霉、奇异翅孢壳、地
生翅孢壳、串珠镰孢、尖镰孢、盘长孢菌(鲁保一号)、银白杨盘长孢、玉蜀黍长蠕
孢、深黄被孢霉、小被孢霉、多头被孢、拉曼被孢霉、葡萄色被孢霉、生香毛霉、卷
枝毛霉、两型孢毛霉、直立毛霉、球孢毛霉、丝球毛霉、大毛霉、多型孢毛霉、总状
毛霉、鲁氏毛霉、五通桥毛霉、黑球漆斑菌、露湿漆斑菌、玫烟色拟青霉、棉铃虫拟
青霉、拟青霉、球形阜孢、白腐菌、少根根霉、华根霉、科恩根霉、戴尔根霉、日本根霉、爪哇根霉、米根霉、点头根霉、绿穂霉、簇孢匍柄霉、雅致枝霉、康宁木霉、绿色木霉、黄萎轮枝孢、大丽花轮枝孢

15. Czapek′s Agar(察氏琼脂)
Sucrose (蔗糖) 30g NaNO3 3g
MgSO4.7H2O 0.5g KCl 0.5g
FeSO4.7H2O 0.01g K2HPO4 1g
Agar (琼脂) 13g Distilled water (蒸馏水) 1000ml
pH 7.2
适用范围:红霉素链霉菌、洋葱曲霉、金头曲霉、泡盛曲霉、泡盛曲霉烟色变种、亮白曲霉、炭黑曲霉、鹿皮色曲霉、棒曲霉、无花果曲霉、黄柄曲霉、黄曲霉、烟曲霉、构巢曲霉、黑曲霉、赭曲霉、米曲霉、寄生曲霉、酱油曲霉、土曲霉、宇佐美曲霉、焦曲霉、杂色曲霉、温特曲霉、阿达青霉、产黄青霉、黄绿青霉、桔青霉、顶青霉、园弧青霉、绳状青霉、灰黄青霉、岛青霉、淡紫青霉、特异青霉、赭绿青霉、产紫青霉、娄地青霉、小刺青霉、荨麻青霉、

16. Concentrated Sucrose Czapek′s (浓糖察氏琼脂)
Sucrose (蔗糖) 200g NaNO3 3g
MgSO4.7H2O 0.5g KCl 0.5g
FeSO4.4H2O 0.01g K2HPO4 1g
Agar (琼脂) 13g Distilled water (蒸馏水) 1000ml
适用范围:谢瓦曲霉

17. Synthetic Potato Medium (综合马铃薯培养基)
20%Potato extract (马铃薯汁) 1L Glucose (葡萄糖) 20g
KH2PO4 3g MgSO4.7H2O 1.5g
Vitanib B1 (硫胺素) Trace (微量) Agar (琼脂) 15g
pH 6
适用范围:米曲霉、酱油曲霉、栖土曲霉、宇佐美曲霉、出芽短梗霉、白僵霉、灰葡萄孢、顶头孢霉、巴西毛客、长刺毛壳、 球毛壳、橄榄包毛壳、反曲毛壳、琥珀毛壳、圆酵毛壳、多主枝孢、束状刺盘孢、葫芦科刺盘孢、新月弯孢霉、奇异翅孢壳、地生翅孢壳、串珠镰孢、蚀脉镰孢霉、盘长孢菌(鲁保一号)、银白杨盘长孢、瓶毛壳、金龟子绿僵菌、生香毛霉、卷枝毛霉、两型孢毛霉、直立毛霉、球孢毛霉、丝球毛霉、大毛霉、多型孢毛霉、总状毛霉、鲁氏毛霉、五通桥毛霉、黑球漆斑菌、露湿漆斑菌、玫烟色拟青霉、棉铃虫拟青霉、淡紫色拟青霉、拟青霉、白腐菌、少根根霉、根霉属的菌、华根霉、科恩根霉、戴尔根霉、台湾根霉、日本根霉、爪哇根霉、黑根霉、雪白根霉、少根根霉、米根霉、点头根霉、三宝垄根霉、根霉属的种、绿穂霉、康宁木霉、木素木霉、拟康氏木霉、木霉、粉红木霉、绿色木霉、黄萎轮枝孢、大丽花轮枝孢、双孢蘑菇、大肥菇、发光假蜜环菌、木耳、皱木耳、毛木耳、大秃马勃、长根菇、金针菇、棘托竹荪、短裙竹荪、短裙竹荪东北变种(暂定)、长裙竹荪、白鬼笔、无裙竹荪、宽鳞大孔菌、凸圆灵芝(白芝)、紫芝、灵芝(红芝,赤芝,日本灵芝,韩国灵芝)、灰树花、猴头菌、香菇、粗毛斗菇、洁丽香菇、豹皮菇、玉蕈、真姬菇、安络小皮伞菌、鬼毛针、黑柄皮伞菌、尖顶羊肚菌、圆锥羊肚菌、高羊肚菌、黑脉羊肚菌、羊肚菌、滑菇、光帽黄伞、尖鳞黄伞、金顶側耳、榆黄磨、金顶磨、白黄側耳、紫孢平菇、姬菇、裂皮側耳、拟囊体側耳(鲍鱼菇)、真线側耳短孢变种、真线側耳、扇形平菇、扁形平菇、佛罗里达无孢子側耳、佛罗里达側耳、蚝菇、糙皮側耳、平菇、贝形側耳、鹰翅菌、肺形側耳、柳树菌、树窝、扁柄側耳、美味側耳、紫孢側耳、粉褶側耳、粉红側耳、凤尾菇、漏斗状側耳、榆干側耳、大榆磨、側耳、茯苓、黑菇、烟色红菇、裂褶菌、鸡纵、金耳、黄木耳、银耳、白木耳、紫晶口磨、银丝菇、丝盖小包脚菇、草菇

18. Corn Meal Medium (玉米粉培养基)
Mix corn meal with water [20:100(W/V)] well . Distribute the mixture into 100ml bottles , each bottle contains about 30ml of the mixture . Autoclave at 121℃ for 30minutes . (20%玉米粉装瓶,15磅灭菌30分钟。)

19. Filter Paper Medium (滤纸培养基)
(NH4)2SO4 1g KH2PO4 1g
MgSO4.7H2O 0.7g NaCl 0.5g
Distilled water (蒸馏水) 1000ml A strip of filter paper (滤纸条) 6ⅹ1cm (厘米)

20. Soybean Cake Meal Agar (黄豆饼粉琼脂)
10% infusion of soybean cake meal (10%黄豆饼粉浸汁) 1000ml
Glucose (葡萄糖) 10g NaCl 2.5g

21. Sawdust, Wheat-bran Medium (木屑、麸皮培养基)
Sawdust of broad leaf tree 75g Wheat bran 25g
Preparation:Mix these two ingredients well . Add appropriate water and blend (the amount of water added may be becided by kneading with fingers ). Fill the mixture into broad mouth of bottles . Stick a hole on the filling, put the cotton pulg in bottle mouth .
壳斗科植物或阔叶树的木屑 75克   麸皮 25克
制法:加水适量充分混匀,水量以手捏时指间有水珠为宜,装瓶并压紧,在中心处扎一小洞,堵上棉塞,20磅灭菌1小时。

22. Pine Block or Pine Sowdust Medium (松木条或松木屑培养基)
1. Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully absorbing suger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr . (将10ⅹ2ⅹ1厘米的松木条浸泡在1-2%的蔗糖水中。使木条吸足糖水,装瓶不宜过紧,用纸包好,20磅灭菌1小时。)
2. Pine Sawdust Medium
Pine Sawdust (松木屑) 75% Rice bran (米糠) 23-24%
Socrose (蔗糖) 1-2% Water (appropriate)水 (适量)
Autoclave at 20 lbs for 1 hr.(20磅灭菌1小时)

23. Rice Straw. Dung Medium (稻草、马粪培养基)
Dried horse dung (or cow dung) (干马粪或牛粪) 65%
Died straw (干稻草) 35% Urea (尿素) 0.5%
Calcium superhosphate (过磷酸钙)0.7% Gypsum powder(石膏粉) 1%
Ammonium sulphate (硫酸铵) 0.3%
[Note]: Add appropriate water, pile them up and cause them fermentation. (水适量进行堆积发酵)
 
24. Straw Rice Bran Medium (稻草、米糠培养基)
Dried straw (干稻草) 75-80% Rice bran (米糠) 20-25%
[Note]:Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12 hr. Allow the straw fully (the amount of water added may be decided by kneading with fingers. It is right when a drop of water appeared between fingers). ( [注]:选无霉稻草,切成长约3厘米的小段,用清水浸泡12小时,使充分吸水,然后倾去水,加米糠和水,水量以用手捏时指间有水珠为宜。)

25. Rice Medium (米饭培养基)
Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoclave at 121℃ for 30 minutes (or fill 2g of rice into a 15ⅹ150mm tube). Add 6 ml of water. Autoclave at 121℃ for 30 minutes. (取大米10克,入50毫升椎形瓶中,水20-30毫升于15磅灭菌30分钟即成,或取大米2克,装入15ⅹ150毫米试管中,加水6毫升,于15磅灭菌30分钟。)

26. Filter Paper Medium (滤纸条培养基)
(NH4)2SO4 1g KH2PO4 1g
MgSO4.7H2O 0.5g Yeast Extract (酵母膏) 0.1g
Distilled Water (蒸馏水) 1000ml
Strip of filter paper 1ⅹ7 cm (滤纸条 1ⅹ7 厘米)
[Note]:Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains 1ml of the broth). Autoclave at 121℃ for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aseptically. (将上述溶液分配成溶液后分装入试管,每管1毫升,15磅灭菌30分钟。再将灭菌的滤纸条(1条或2条)投入试管中即好使用。)

27. Yeast Extract-Medium Extract Agar I (酵母膏、麦芽膏琼脂)
Yeast extract (酵母膏) 10g Mall extract (麦芽膏) 10g
Glucose (葡萄糖) 4g Distilled water (蒸馏水) 1000ml
Agar (琼脂) 20g pH 7.0
适用范围:红霉素链霉菌

28. Yeast Extract Peptone (酵母膏、蛋白胨琼脂)
Yeast extract (酵母膏) 1g Multi-peptone(多蛋白胨) 2g
Beef extract (牛肉膏) 1g Glucose (葡萄糖) 10g
Agar (琼脂) 20g Distilled water (蒸馏水) 1L
pH 7.0

29. Glucose Yeast Extract Starch Agar (葡萄糖、酵母、淀粉琼脂)
Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g
Starch (淀粉) 10g NaCl 5g
CaCO3 3g Agar (琼脂) 20g
Distilled water (蒸馏水) 1L pH 7.0

30. Wheat Bran Medium (麸皮培养基)
Wheat bran (麸皮) 36g (NH4)2HPO4 10g
K2HPO4 0.2g MgSO4。7H2O 0.1g
Agar (琼脂) 20g Distilled water (蒸馏水) 1L
pH 7.0

31. Yeast Sporulation Medium (酵母菌产生子囊孢子培养基)
(1) Kleyn Medium
KH2PO4 0.012% K2HPO4 0.02%
Sodium acetate (醋酸钠) 0.5% Glucose (葡萄糖) 0.062%
NaCl 0.062% Peptone (蛋白胨) 0.25%
Biotin (生物素) 2μg% Agar (琼脂) 2%
Trace element solution (混合盐类) 1ml/100ml
[Note]: Trace element solution (混合盐类溶液): MgSO4.7H2O 0.4%, NaCl 0.4%, CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2%
(2) Gorodkowa Medium
Glucose (葡萄糖) 0.1% Peptone (蛋白胨) 1%
NaCl 0.5% Agar (琼脂) 2%
(3) McClary Medium
Glucose (葡萄糖) 0.1% KCl 0.18%
Yeast juice (酵母汁) 0.25% Sodium actate (醋酸钠) 0.82%
Agar (琼脂) 1.5%

32. BPY Medium (BPY 培养基)
Beef extract (牛肉膏) 5g Prptone (蛋白胨) 10g
NaCl 5g Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 5g Agar (琼脂) 1-2%
Distilled (蒸馏水) 1000ml pH 7.0
适用范围:大肠埃希氏菌(大肠杆菌)、恶臭假单胞菌

33. LB Medium (LB 培养基)
Yeast extact (酵母膏) 5g Peptone (蛋白胨) 10g
NaCl 10g Agar (琼脂) 1-2%
Distilled water (蒸馏水) 1000ml pH 7.0
适用范围:大肠埃希氏菌(大肠杆菌)

34. Penssay Broth (Penssay 肉汤)
Beef extract (牛肉膏) 1.5g Yeast extract (酵母膏) 1.5g
Tryptone (胰蛋白胨) 5g NaCl 3.5g
Glucose (葡萄糖) 1g K2HPO4.3H2O 4.8g
Distilled water (蒸馏水) 1000ml pH 7.2

35. Blond Agar (血琼脂培养基)
Peptone (蛋白胨) 10g Glucose (葡萄糖) 2g
NaCl 5g NaH2PO4 2.5g
Sodium citrate (柠檬酸钠) 5g Brain infusion (脑浸汁) 800ml
Pig heart infusion (猪心浸) 200ml Sleep Blood (羊血) 10%
pH 7.2
[Note]:Preparation of pig brain and pig heart infusion:
(1) Smash up 100g fresh pig heart (without fat and blood vessel).Add distilled water up to 1000ml;
(2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml;
(3) Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes;
(4) Cool and filtrate through gauze . [注]猪心和猪脑浸汁法:(1)新鲜猪心除去心头和脂肪,搅碎100克加蒸馏水至1000毫升;(2)新鲜猪脑除去血管,取200克加蒸馏水至1000毫升;(3)50℃保温1小时,然后煮开15分钟;(4)冷却后用纱布过滤备用。

36. Cellulose﹣decomposing Bacteria Synthetic Medium (纤维细菌合成培养基)
NaCl 6g MgSO4.7H2O 0.1g
KH2PO4 0.5g CaCl2 0.1g
K2HPO4 2.0g Yeast extract (酵母膏) 1.0g
(NH4)2SO4 2.0g Distilled water (蒸馏水) 1000ml
Filter paper strip: one strip (滤纸条:一片) pH 7.0-7.4
[Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. (斜面培养基去滤纸条用2%琼脂及2%羧甲基纤维素钠 CMCNa )

37. Clostridium Pasreurianum Synthetic Medium (巴氏梭状芽孢杆菌合成培养基)
Glucose (葡萄糖) 1% MnSO4 0.001%
KH2PO4 0.05% FeSO4 0.001%
K2HPO4 0.05% Yeast extract(酵母膏) 0.1%
MgSO4.7H2O 0.02% Peptone (蛋白胨) 0.01%
NaCl 0.001% CaCO3 0.5%
Distille water (蒸馏水) 100% pH 7.0

38. Yeast Extract-Medium Extract Agar Ⅱ (酵母膏、麦芽膏琼脂 Ⅱ)
Yeast extract (酵母膏) 4g Mall extract (麦芽膏) 10g
Glucose (葡萄糖) 4g Agar (琼脂) 17g
Distilled water (蒸馏) 1000ml pH 7.3
39. Oatemeal Agar (燕麦粉琼脂)
Oatemeal(infusion)[燕麦粉浸液] 20g Agar (琼脂) 18%
Trace element solution (迹量盐溶液)1ml
Distilled water up to (用蒸馏水补充至1000毫升) 1000ml pH 7.2
[Note]:Trace element solution (迹量盐溶液):
FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g
ZnSO4.7H2O 0.1g Distilled water (蒸馏水) 100ml

40. Calcium Malate Agar (苹果酸钙琼脂)
Calium malate (苹果酸钙) 10g K2HPO4 0.5g
NH4Cl 0.5g Glycerol (甘油) 10g
Agar (琼脂) 15g Distilled water (蒸馏水) 1000ml

41. Thiobacillus Ferrooxidans Medium (氧化亚铁硫杆菌培养基)
(NH4)2SO4 0.15g KH2PO4 0.05g
KCl 0.05g MgSO4.7H2O 0.5g
Ca(NO3)2.4H2O 0.01g FeSO4.7H2O 50g
Distilled water (蒸馏水) 1000ml pH 2.0

42. Thiobacillus Thiooxidans Medium (氧化硫硫杆菌培养基)
(NH4)2SO4 0.3g KH2PO4 3-4g
CaCl2 (anhydrous) [无水] 0.25g MgSO4.7H2O 0.5g
FeSO4.7H2O 0.001g Distilled water (蒸馏水) 1000ml
pH 3.5-4

43. Plasma Substitute Medium (代血浆培养基)
Sucrose (蔗糖) 12.5-15% Peptone (蛋白胨) 0.5%
KH2PO4 0.03% Na2HPO4.12H2O 0.14%
pH 7.0-7.2

44. Skim Milk Medium (脱脂牛奶培养基) ATCC 183
脱脂牛奶 100g 去离子水 1000ml
适用范围:保加利亚乳杆菌、嗜热乳酸链球菌

45. Pea Agar (豌豆琼脂)
Glucose (葡萄糖) 1% Peptone (蛋白胨) 0.5%
NaCl 0.5% Agar (琼脂) 2.0%
Pea infusion (counted by NH2) [豌豆浸汁(以NH2计)] 12-14 mg∕100
pH (before sterilization) [消前] 7.2-7.5
[Note]: Preparetion of pea infusion (豌豆浸汁的制法) :
Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37℃ for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Distribution and autoclave at 120℃ for 30 minutes. Store at cool contition . (称取白豌豆900克,置于5公升广口瓶中,加自来水3公升,用硫酸调pH至4.3-4.5,再加氯仿30毫升(防腐),加胶塞并用纱布扎口(防止冲脱),放37℃恒温室7-10天。取出用滤纸过滤,滤液煮沸20分钟,以除氯仿,并再次以滤纸过滤,除沉淀。滤液分装,于120℃30分钟灭菌。后冷藏备用。)
The standard quality of pea infusion as following (豌豆浸汁质量标准):
Aminonitrogen(氨基酸): 160-200 mg∕100ml
Soluble phosphoruse(溶磷): 400-600 μg∕ml
Color(色泽): auratus,transparent (金黄、透明)

46. Wheat Bran Medium (麦麸琼脂)
Wheat bran (麦麸) 3.6% (NH4)2HPO4 0.3%
K2HPO4 0.02% MgSO4.7H2O 0.01%
Agar (琼脂) 2% pH 7.0
适用范围:金霉素链霉菌、红霉素链霉菌

47. Glucose Yeast Extract Agar (葡萄糖、酵母膏琼脂)
Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g
Water (水) 1000ml Agar (琼脂) 15g
pH 7.2

48. MMN Medium (MMN培养基)
CaCl2 0.05g MgSO4 0.15g
NaCl 0.025g FeCl3 (1%) 1.2ml
KH2PO4 0.5g Vitamib B1 (硫胺素) 100μg
(NH4)2HPO4 0.25g Wort (12 Be′) 100ml
Glucose (葡萄糖) 10-15g Citric acid (柠檬酸) 0.2g
Distilled water (蒸馏水) 900ml pH 5.5 (左右)
适用范围:蜜环菌

49. Starch Agar (淀粉琼脂)
Soluble starch (可溶性淀粉) 10g NaNO3 1g
MgCO3 1g K2HPO4 0.3g
NaCl 0.5g Agar (琼脂) 20g
Distilled water (蒸馏水) 1000ml

50. 5 Be′Wort Agar (5 Be′麦芽汁琼脂)
适用范围:园弧青霉、少根根霉、日本根霉、爪哇根霉

51. 5 Be′Koji Extract Agar (5 Be′米曲汁琼脂)
适用范围:华根霉、三宝垄根霉

52. 发根农杆菌培养基(YEB)
Beef extract (牛肉浸膏) 5g/L Yeast extract (酵母膏) 1g/L
Peptone (蛋白胨) 5g/L Sucrose (蔗糖) 5g/L
MgSO4.7H2O 0.4g/100ml Agar (琼脂) 1.5g/100ml
pH 7.4
适用范围:青枯假单胞菌

53. 青枯菌培养基 (Tm)
Peptone (蛋白胨) 10g Casein (干酪素水解物产) 1g
Glucose (葡萄糖) 5g Ddhro (纯水) 1000ml
Agar(琼脂) 15g pH 7.0

54. YM (Yeast Malt Agar)
酵母膏 3.0g 麦芽汁 3.0g
蛋白胨 5.0g 琼脂 20.0g
蒸馏水 1000ml

55. ISP 4
可溶性淀粉 10.0g K2HPO4 1.0g
MgSO4.7H2O 1.0g NaCl 1.0g
(NH4)2SO4 2.0g CaCO3 2.0g
FeSO4.7H2O 0.001g MnCl2.7H2O 0.001g
琼脂 20.0g 蒸馏水 1000ml

56. Trypton Soy Agar
胰蛋白胨 17.0g 大豆胨 3.0g
葡萄糖 2.5g 琼脂 20.0g
NaCl 5.0g K2HPO4 2.5g
蒸馏水 1000ml

57. NA (Nutrient Agar)
牛肉膏 3.0g 蛋白胨 5.0g
琼脂 20.0g 蒸馏水 1000ml
pH 6.6-7.0

58. 甘露(糖)醇琼脂 (ATCC 1)
酵母膏 5.0g 蛋白胨 3.0g
甘露(糖)醇 25.0g 琼脂 15.0g
蒸馏水 1000ml

59. Marine琼脂 2216 (ATCC 2)
蛋白胨 5.0g 酵母膏 1.0g
柠檬酸铁 0.1g 溴化钾 0.08g
NaCl 19.45g MgCl2 8.8g
Na2SO4 3.24g CaCl2 1.8g
KCl 0.55g NaHCO3 0.16g
NH4NO3 0.0016g Na2HPO4 0.008g
氯化锶 0.034g 硼酸 0.022g
硅酸钠 0.004g 氟化钠 0.0024g
琼脂 15.0g 蒸馏水 1000ml
pH 7.4-7.8

60. 兔血琼脂(ATCC 4)
牛心(取其浸液) 500.0g 胰蛋白胨 (Tryptose) 10.0g
NaCl 5.0g 琼脂 15.0g
蒸馏水 1000ml pH 6.6-7.0

61. 孢子形成培养基 (ATCC 5)
酵母膏 1.0g 牛肉膏 1.0g
胰蛋白胨 2.0g 葡萄糖 10.0g
FeSO4 微量 琼脂 15.0g
蒸馏水 1000ml pH 7.2

62. Trypticase Soy Agar (ATCC 18)
Trypticase Soy Broth 30.0g 琼脂 15.0g
蒸馏水 1000ml
63. YPAD (ATCC 109)
酵母膏 10.0g 蛋白胨 20.0g
葡萄糖 20.0g 琼脂 20.0g
蒸馏水 1000ml pH 7.0

64. SP (ATCC 432)
棉子糖 1.0g 蔗糖 1.0g
半乳糖 1g 可溶性淀粉 5g
水解酪蛋白 2.5g 琼脂 20.0g
MgSO4.7H2O 0.5g K2HPO4 0.25g
蒸馏水 1000ml pH 自然

65. 胰酪胨大豆酵母浸膏琼脂 (TSA-YE)
胰蛋白胨 15.5g 大豆蛋白胨 5.0g
NaCl 5.0g 酵母膏 6.5g
琼脂 15.0g 蒸馏水 1000ml
pH 7.3

66. ATCC 20
蛋白胨 5.0g 酵母膏 15.0g
K2HPO4 3.0g 葡萄糖 2.0g
琼脂 20.0g 蒸馏水 1000ml
pH 7.3-7.5

67. TY (Yeast Tryptone Agar)
蛋白胨 10.0g 酵母膏 5.0g
葡萄糖 1.0g NaCl 5.0g
琼脂 20.0g 蒸馏水 1000ml
pH 7.0

68. 硫醇(Thiol)培养基 (ATCC 49)
示蛋白胨(Proteose Peptone No.3,Difco) 10.0g 酵母膏 5.0g
葡萄糖 1.0g NaCl 5.0g
硫醇复合物 8.0g 琼脂 1.0g
p-氨基苯甲酸 0.05g
pH 6.9-7.3

69. Czapek'Dox 琼脂培养基 (ATCC 134)
Czapek'Dox Broth 35.0g 琼脂 15.0g
蒸馏水 1000ml

70. MG/L
蛋白胨 5.0g 甘露醇 5.0g
谷氨酸钠 1.15g 生物素 0.0001g
K2HPO4 0.25g NaCl 0.1g
MgSO4.7H2O 0.1g 酵母膏 2.5g
琼脂 20.0g 蒸馏水 1000ml
氯霉素(终浓度) 50μg/ml
pH 7.0

71. Eclb Broth
蛋白胨 10.0g 酵母膏 5.0g
NaCl 5.0g 琼脂 20.0g
蒸馏水 1000ml 氨苄青霉素(终浓度) 300μg/ml
pH 7.0

72. Bennetts Agar (IFO 228)
酵母膏 1.0g 牛肉膏 1.0g
NZ Amine,type A 2.0g 葡萄糖 10.0g
琼脂 20.0g 蒸馏水 1000ml
pH 7.3

73. Harroldˊs M40Y (ATCC 319)
麦芽膏 20.0g 酵母膏 5.0g
蔗糖 400.0g 琼脂 20.0g
蒸馏水 1000ml

74. PSA (Potato Sucrose Agar)
土豆 200g 蔗糖 20g
蒸馏水 1000ml 琼脂 20g
pH 6.0-6.5

75. 培养基
牛肉膏 300g 水解酪蛋白 17.5g
可溶性淀粉 1.5g 琼脂 20.0g
蒸馏水 1000ml 磺胺嘧啶 (终浓度) 100μg/ml
氨苄青霉素 (终浓度) 100μg/ml
pH 7.3-7.4

76. 马铃薯牛肉汁培养基
麦芽糖 20.0g 牛肉膏 5.0g
蛋白胨 3.0g 马铃薯 200.0g
pH 7.0-7.2

77. ISP 2
酵母膏 4.0g 麦芽汁 10.0g
葡萄糖 4.0g 琼脂 20.0g
蒸馏水 1000ml
pH 7.0

78. BHI (Brain Heart Infusion)
牛脑 200.0g 牛心浸出汁 250.0g
蛋白胨 10.0g 葡萄糖 2.0g
NaCl 5.0g 琼脂 20.0g
蒸馏水 1000ml
pH6.8-7.2

79. YPG (Yeast Peptone Glucose)
酵母膏 10.0g 蛋白胨 20.0g
葡萄糖 20.0g 琼脂 20.0g
蒸馏水 1000ml pH 7.0

80. 兔食琼脂培养基(ATCC 340)
兔食(商店出售) 25.0g 琼脂 20.0g
蒸馏水 1000ml
*煮沸兔食,并浸泡1/2小时,用多层纱布过滤,加琼脂于滤液中。

81. 土豆琼脂(ATCC 423)
200克去皮土豆切成小块于1000ml自来水中煮沸1小时,用细棉布过滤.加进5.0mgMnSO4,pH调至6.8,用自来水定容至1000ml。加进15.0g琼脂,溶化,分装并灭菌。
 
82. 土豆浸汁加无机盐培养基(ATCC 470)
土豆 2000g MnSO4.5H2O 0.02g
MgSO4.7H2O 0.4g ZnSO4.7H2O 0.01g
CuSO4. 5H2O 0.01g FeSO4.7H2O 0.1g
CaCl2.2H2O 0.1g K2HPO4 0.5g
* 称取200g去皮块状土豆,加入1000ml蒸馏水煮沸30分钟,多层纱布过滤,滤液中加入以上成分,溶解后定容至1000ml。
83. 无机盐淀粉琼脂培养基(ATCC 527)
可溶性淀粉 10.0g K2HPO4 1.0g
MgSO4 1.0g NaCl 1.0g
(NH4)2SO4 2.0g CaCO3 2.0g
FeSO4.7H2O 0.001g MnCl2.7H2O 0.001g
ZnSO4.7H2O 0.001g 琼脂 20.0g
pH 7.0-7.4

84. (AS125)
新鲜鸡蛋 15-16 蒸馏水 375ml
2%孔雀(石)绿 10.0g K4HPO4 1.5g
MgSO4.7H2O 0.375g 天冬酰胺 2.25g
甘油 7.5ml 土豆粉 18.8g

85. 燕麦片(Oatmeal)琼脂培养基
燕麦片 60.0g 琼脂 12.5g
蒸馏水 1000ml
* 将燕麦片加600ml水中,制成匀浆,加热至45-50oC,然后加入已溶化琼脂的另400ml水,121oC灭菌90分钟。

86. 细菌培养基(ATCC 573)
(NH4)2SO4 1.3g K2HPO4 0.37g
MgSO4.7H2O 0.25g CaCl2.2H2O 0.07g
FeCl3 0.02g 葡萄糖 1.0g
酵母膏 1.0g 蒸馏水 1000ml
用10N H2SO4 调pH至4.0。若配制固体培养基,为增强上述溶液的强度,pH需调至3.5,琼脂水溶液浓度为40g/L。两者分别高压灭菌,冷却至约50oC,无菌操作将两种溶液等体积混合,这一过程可避免琼脂被酸水解。

87. 碎肉培养基(ATCC 593)
牛肉(去脂肪) 500.0g 蒸馏水 1000ml
NaOH 25.0ml
* 所用瘦牛肉或马肉(碾)磨碎前需去脂肪和周围的组织。将肉、水、NaOH混合后边搅拌边加热,冷至室温,去掉表面油脂,过滤,留下肉末和滤液。滤液加蒸馏水至1000ml。滤液中加下列成分:
蛋白胨 30.0g 酵母膏 5.0g
K2HPO4 5.0g 0.025%刃天青(Resazurin)溶液4.0ml
然后煮沸,冷却,加0.5g L-半胱氨酸HCl。H2O,pH调至7.0。在氧气、氮气(O2-freenitrogen)和3%氢气条件下,按1份碎肉加4-5份以上溶液的比例分装于每支试管。在氮气和氢气条件下,使用橡皮塞,快排气,高压灭菌15分钟。
88. YMPG
酵母膏 3.0g 麦芽汁 3.0g
蛋白胨 5.0g 葡萄糖 10.0g
FeSO4.7H2O 0.1g 琼脂 20.0g
蒸馏水 1000ml pH 6.8
灭菌:110oC,35分钟
89. TGYM培养基(ATCC 679)
胰蛋白胨 5.0g 葡萄糖 1.0g
酵母膏 3.0g 蒸馏水 1000ml
DL-蛋氨酸(DL-Meth 0.5g
90. TYG培养基(ATCC 741)
胰蛋白胨 3.0g 酵母膏 3.0g
葡萄糖 3.0g K2HPO4 1.0g
琼脂 20.0g 蒸馏水 1000ml
pH 7.4

91. M9
Na2HPO4 12.8g KH2PO4 3.0g
NaCl 0.5g NH4Cl 0.5g
葡萄糖(单独灭菌) 10.0g 维生素B1(单独灭菌) 1μg/ml
pH 自然
92. ISP3 (IFO 245)
燕麦粉 20.0g 微量元素溶液 1ml
pH 7.2
微量元素:
FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g
ZnSO4.7H2O 0.1g
93. Oatmeal Agar
琼脂 5.0g 蒸馏水 500ml
加热溶化速溶燕麦粉 40.0g 蒸馏水 250ml
加热溶化后与琼脂液混合加水至1000ml pH 5.8
94. V8-Juice Agar (AYCC 343)
V-8 果汁 200g CaCO3 3.0g
琼脂 20.0g 蒸馏水 1000ml
pH 5.8
95. Czapek-Dox Agar (ATCC 312)
NaNO3 3.0g K2HPO4 1.0g
MgSO4.7H2O 0.5g KCl 0.5g
FeSO4.7H2O 0.01g 蔗糖(单独灭菌) 30.0g
琼脂 20.0g 蒸馏水 1000ml
pH 5.6
96. GC培养基 (ATCC 814)
A 液
琼脂 5.0g GC琼脂培养基(BBL 11275) 36.0g
蒸馏水 500.0ml
B 液
干燥色牛血红蛋白(BBL 11871) 10.0g 蒸馏水 500.0ml
Iso Vitale X (BBL 11876) 10.0ml
97. 固氮螺菌培养基 (ATCC 838)
K2HPO4 0.1g KH2PO4 0.4g
MgSO4.7H2O 0.2g NaCl 0.1g
CaCl2 0.02g FeCl3 0.01g
NaMoO4.2H2O 0.002g 苹果酸钠(Na malate) 5.0g
酵母膏 0.05g 蒸馏水 1000ml
pH 7.2-7.4
98. BG-11
NaNO3 1.5g K2HPO4 0.04g
MgSO4.7H2O 0.075g CaCl2.7H2O 0.036g
Na2CO3 0.02g 柠檬酸 0.006g
柠檬酸铁 0.006g *微量元素溶液 A5 1ml
氨苄青霉素 (终浓度) 50μg/ml 蒸馏水 1000ml
琼脂 20.0g
* 微量元素溶液 A5
H3BO4 2.86g MnCl2.4H2O 1.81g
ZnSO4 0.222g Na2MoO4 0.39g
CuSO4.5H2O 0.079g Co(NO3)2.6H2O 49.4g
99. (Marine Agar)
蛋白胨 5.0g 酵母膏 1.0g
柠檬酸铁 0.1g NaCl 19.45g
MgSO4.7H2O 5.9g KCl 0.55g
Na2SO4 3.24g CaCl2 1.8g
NaHCO3 0.16g KI 0.08g
微量元素 10ml pH 7.0-7.6
* 微量元素溶液:
氯化锶 0.034g 硅酸钠 0.004g
蒸馏水 1000ml H3BO4 0.022g
NaCl 0.0024g NaNO3 0.0016g
NaHPO4 0.008g 蒸馏水 1000ml
100.(Yeast Starch Agar)
酵母膏 2.0g 可溶性淀粉 10.0g
琼脂 20.0g 蒸馏水 1000ml
pH 7.2-7.4
101.GYMC
葡萄糖 4.0g 酵母膏 2.0g
麦芽汁 20.0g 琼脂 20.0g
CaCO3 1.5g 蒸馏水 1000ml
pH 6.8-7.0
102. LCSB
乳糖 15.0g 玉米浆 5.0g
蛋白胨 5.0g NaCl 4.0g
MgSO4.7H2O 0.5g KH2PO4 0.6g
FeCl3.6H2O 0.005g CuSO4.5H2O 0.002g
琼脂 20.0g 蒸馏水 1000ml
相对湿度 65% pH 4.8
103.高氏1号(G)
可溶性淀粉 20.0g KNO3 1.0g
K2HPO4 0.5g MgSO4.7H2O 0.5g
NaCl 0.5g FeSO4.7H2O 0.01g
琼脂 20.0g 蒸馏水 1000ml
pH 7.0-7.2
104.葡萄糖-酵母膏琼脂(ATCC 846)
酵母膏 5.0g 蛋白胨 1.0g
KH2SO4 1.0g NaCl 1.0g
琼脂 20.0g 蒸馏水 950.0ml
pH 6.8
补加50ml10%葡萄糖(葡萄糖溶液单独过滤灭菌)。
105.Tep尿液(Uric Acid)琼脂(ATCC 894)
Na2HPO4.12H2O 9.0g KH2PO4 1.5g
MgSO4.7H2O 0.2g 柠檬酸铁铵(绿色) 1.2g
CaCl2 20.0mg MnCl2.4H2O 1.0mg
Trypticcase Soy Broth (BBL 11768) 3.0g 尿素 4.0g
琼脂 20.0 蒸馏水 1000ml
pH 7.2
106.增强梭菌生长的培养基 (ATCC 1053)
胰蛋白胨 10.0g 牛肉膏 10.0g
酵母膏 3.0g 葡萄糖 5.0g
NaCl 5.0g 可溶性淀粉 1.0g
半胱氨酸盐酸盐 0.5g 醋酸钠 3.0g
琼脂 15.0g 蒸馏水 1000ml
pH 6.6-7.0
107.酵母膏-甘露(糖)醇琼脂 (ATCC 1205)
K2HPO4 0.5g MgSO4.7H2O 0.2g
NaCl 0.1g 甘露(糖)醇 10.0g
酵母膏 0.4g 蒸馏水加至 1000ml
108.Tomato Dextrin Agar (ATCC 965)
番茄汁 20.0g 糊精 20.0g
酵母膏 10.0g CoCl2.6H2O 5.0mg
琼脂 20.0g 蒸馏水 1000ml
pH 7.2-7.4
109.嗜热菌无机盐溶液 (ATCC 1554)
FeSO4 1.0mg MgSO4.7H2O 200.0mg
Na2HPO4 210.0mg NaH2PO4 90.0mg
KCl 40.0mg CaCl2 15.0mg
NaNO3 0.25g NH4Cl 0.25g
微量元素溶液(见下面) 10.0ml
n-Heptadecane(C17) 0.1%(v/v) 蒸馏水 1000ml
微量元素溶液:
CuSO4.5H2O 500mcg H3BO4 1.0mg
MnSO4.5H2O 7.0mcg ZnSO4.7H2O 7.0mg
MoO3 1.0mg CoSO4.7H2O 18.0mcg
蒸馏水 1000ml
110.醋杆菌培养基 (AS 1)
葡萄糖 100.0g 酵母膏 10.0g
CaCO3 20.0g 琼脂 15.0g
蒸馏水 1000ml pH 6.8

111. 葡萄糖天冬酰胺琼脂培养基 (AS 11)
葡萄糖 10.0g 天冬酰胺 (Asparagine) 0.5g
K2HPO4 0.5g 蒸馏水 1000ml
pH 7.2-7.4
112.麦芽汁琼脂 (AS13)
稀释去除啤酒花的麦芽汁至浓度为12Brix(白利-糖浓度单位)。按1.5%的比例将琼脂加进上述麦芽汁中,加热溶解,然后将此培养基分装试管中。高压灭菌:110℃,30分钟。
113.(ATCC 805)
酵母膏 1.0g K2HPO4 0.7g
MgSO4.7H2O 1.0g 甘露糖醇 5.0g
KH2PO4 0.1g 蒸馏水 1000ml
琼脂 20.0g pH 7.4
114.Todd-Hewitt Medium
牛心脏 500.0g 蛋白胨 20.0g
葡萄糖 2.0g NaCl 2.0g
Na2HPO4 0.4g Na2CO3 2.5g
琼脂 20.0g 蒸馏水 1000ml
pH 7.8
115.MSK
酵母膏 1.0g 脱脂奶粉 100.0g
琼脂 20.0g 蒸馏水 1000ml
pH 6.9
116.土豆葡萄糖琼脂培养基 (AS 14)
土豆 500g 葡萄糖 20.0g
琼脂 20.0g
* 称取500克土豆,去皮切成丁,立即加入1000ml水,充分煮沸,用棉布过滤。滤液用水定容至1000ml,再加入其它成分。高压灭菌:115℃,20分钟。
117.PCA (ATCC 343)
土豆 20.0g 胡萝卜 20.0g
琼脂 20.0g 蒸馏水 1000ml
pH 5.8
118.(Mueller-Hinton Agar)
牛肉膏 300.0g 水解酪蛋白 17.5g
淀粉 1.5g 磺胺嘧啶(终浓度) 50μg/ml
琼脂 20.0g 蒸馏水 1000ml
氨苄青霉素(终浓度) 50μg/ml
pH 7.2
119.豆饼粗粉琼脂培养基 (AS 20)
10%豆饼粗粉浸液 1000g 葡萄糖 10.0g
NaCl 2.5g 琼脂 15.0g
120.北京棒状杆菌噬菌体培养基 (AS 48)
(1)牛肉膏 3.0g 蛋白胨 5.0g
NaCl 5.0g 葡萄糖 10.0g
蒸馏水 1000ml pH 7.0
(2)葡萄糖 20.0g 玉米浆 8.0g
K3PO4.3H2O 1.0g MgSO4.7H2O 0.4g
尿素(Urea) 5.0g Fe+,Mn+分别为 2 ppm
蒸馏水 1000ml pH 7.0

121. 营养肉汤和葡萄糖培养基 (AS 100)
牛肉膏 10.0g 蛋白胨 10.0g
葡萄糖 10.0g NaCl 5.0g
琼脂 15.0-20.0g 蒸馏水 1000ml
pH 7.0
122.(Trypticase Soya Agar)
胰蛋白胨 17.0g 大豆胨 3.0g
葡萄糖 2.5g NaCl 5.0g
K2HPO4 2.5g 甘油(50%)
琼脂 20.0g 蒸馏水 1000ml
pH 7.0
123.尿囊素无机盐培养基 (DSM 6)
K2HPO4 0.8g KH2PO4 0.2g
MgSO4.7H2O 0.5g CaCl2.2H2O 0.05g
FeSO4.7H2O 0.01g MnSO4.H2O 1.0mg
尿囊素(Allantion) 20.0g 琼脂 15.0g
蒸馏水 1000ml
124.碱性营养琼脂培养基 (DSM 31)
蛋白胨 5.0g 牛肉膏 3.0g
琼脂 20.0g 蒸馏水 1000ml
灭菌后,加入无菌的1M Na - Sesquicar - bonate溶液:(1:10v/v),至pH9.7。
Na - Sesquicarborbonate 溶液:
NaHCO3 4.2g Na2CO3 5.3g
蒸馏水 100.0ml
125.Trypticase Soy 琼脂培养基(DSM 220)
酪蛋白胨(Peptone from casein)5.0g 豆粉蛋白胨(Peptone from soymeal)5.0g
NaCl 5.0 琼脂 15.0g
蒸馏水 1000ml pH 7.3
126.(IFO 203)
蛋白胨 10.0g 酵母膏 5.0g
肝浸出液 25.0g 葡萄糖 3.0g
甘油(Glycerol) 15.0g NaCl 3.0g
琼脂 20.0g 蒸馏水定容至 1000ml
pH 7.2
* Wako Pure Chemicals Ind.Lte.,Osaka Japan.
127.(IFO 231)
酵母膏 1.0g 牛肉膏 1.0g
NZ胺(Amine),typeA 2.0g 麦芽糖(Maltose) 10.0g
琼脂 20.0g 蒸馏水 1000ml
pH 7.3
* Sheffield Chemical Co.,San Ramon,CA 94583,USA or wako Pure Chemical Ind.Ltd.,Osaka,Japan.
128.胰酪胨大豆酵母浸膏琼脂 (TSA - YE)
胰蛋白胨 15.5g 大豆蛋白胨 5.0g
NaCl 5.0g 酵母膏 6.5g
琼脂 15.0g 蒸馏水 1000ml
pH 7.1-7.5
129.MRS
蛋白胨 10.0g 牛肉膏 10.0g
酵母膏 5.0g 葡萄糖 10.0g
琼脂 20.0g 蒸馏水 1000ml
pH 6.5
130.(SEM)
蔗糖 10.0g K2HPO4 0.5g
MgSO4.7H2O 0.2g MgCl2 0.2g
CaCO3 1.0g 酵母膏 0.4g
琼脂 20.0g 蒸馏水 1000ml
pH 7.0-7.2
注:用无水MgSO4量减半

131.(无氮M)
酵母膏 1.0g 土壤浸汁液 200g
琼脂 20.0g 蒸馏水 800g
甘露醇 10.0g pH 7.2
土壤浸汁液制法:取沃土50g加水200ml,15磅灭菌1小时,过滤取滤液,加水到200ml。
132.(BPY)
牛肉膏 5.0g 酵母膏 5.0g
葡萄糖 5.0g 蛋白胨 10.0g
NaCl 5.0g 琼脂 20.0g
蒸馏水 1000ml pH 7.2
133.M 17
胰蛋白胨 50.0g 大豆胨 50.0g
牛肉膏 50.0g 酵母膏 25.0g
抗坏血酸 5.0g MgSO4.7H2O 2.5g
蔗糖 10.0g 磷酸甘油二钠 190.0g
琼脂 20.0g 蒸馏水 1000ml
pH 6.9
134.(乳酸钠 M)
乳酸钠 10.0g K2HPO4 1.67g
MgSO4.7H2O 0.1g KH2PO4 0.87g
NaCl 0.05g CaCl2 40mg
FeCl3 4mg 酵母膏 1.0g
琼脂 20.0g 蒸馏水 1000ml
pH 6.8
135.RVVBN
苹果酸 4.0g (NH4)2SO4 1.0g
MgSO4.7H2O 120mg CaCl2.2H2O 75mg
Na2EDTA 20mg 磷酸缓冲液(钾盐) 10mM
生物素 15μg 维生素B1 1mg
微量元素溶液 1ml
* 微量元素溶液:
HBO3 0.7g MnSO4.H2O 398mg
ZnSO4.7H2O 60mg NaMoO.2H2O 188mg
Ca(NO3)2.3H2O 10mg NaCl 3.0g
蒸馏水 250ml

136.R2YE,R5
蔗糖 103.0g K2SO4 0.25g
MgCl2.6H2O 10.12g 葡萄糖 10.0g
水解酪蛋白 0.1g 微量元素溶液 2.0g
酵母膏 5.0g TES缓冲液 57.3ml
KH2PO4(0.5%) 10ml CaCl2.2H2O(5M) 4ml
L-脯氨酸(20%) 150ml NaOH(1N) 7ml
琼脂 20.0g 蒸馏水 1000ml
pH 7.2
* 微量元素溶液(每升)
ZnCl2 40mg FeCl2.6H2O 200mg
CuCl2.2H2O 10mg MnCl2.4H2O 10mg
Na2B4O7.10H2O 10mg (NH4)6Mo7O24.4H2O 10mg
后4种试剂单独灭菌,使用时加入。
137.MRS (DSM 11)
水解酪蛋白 10.0g 酵母膏 5.0g
葡萄糖 20.0g 吐温80 1.0g
柠檬酸铵 2.0g K2PO4 2.0g
NaAc 2.0g MnSO4.7H2O 0.2g
MgSO4 0.05g 琼脂 20.0g
蒸馏水 1000ml pH 6.2-6.5
138.ISP5 (IFO 265)
L-天门冬氨酸 1.0g 甘油 10.0g
K2HPO4 1.0g 微量元素溶液 1ml
琼脂 20.0g 蒸馏水 1000ml
pH 7.2
* 微量元素溶液:
FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g
ZnSO4.7H2O 0.1g
139. 羧基纤维素酵母培养基
羧甲基纤维素 15.0g 酵母浸膏 1.0g
MgSO4.7H2O 0.5g KH2PO4 1.0g
琼脂 20.0g 蒸馏水 1000ml
* 各成分依次溶于水中,121℃灭菌15分钟
140.(ATCC 464)
蛋白胨 5.0g 胰蛋白胨 5.0g
酵母膏 5.0g 葡萄糖 5.0g
琼脂 20.0g 蒸馏水 1000ml

141.(IFFI 24 M)
牛肉膏 5.0g 酵母膏 5.0g
蛋白胨 10.0g 葡萄糖 10.0g
乳糖 5.0g NaCl 5.0g
琼脂 20.0g 蒸馏水 1000ml
pH 6.8
142.(IFFI 8 M)
麦芽汁或麦芽浸膏 3.0g 酵母膏 5.0g
CaCO3 6.0g 蒸馏水 1000ml
琼脂 20.0g pH自然
143. 土壤浸汁-蛋白胨牛肉膏培养基(ATCC 654)
蛋白胨 5.0g 牛肉膏 3.0g
琼脂 15.0g 土壤浸汁 1000ml
土壤浸汁:取干燥去杂石的土壤400g加入960ml自来水中。121℃高压灭菌1小时,到达压力后,至少保压30分钟。棉布初滤,再用滤纸过滤。再次灭菌121℃,20分钟。滤液用于配制培养基。
144.(AS 95)
牛肉膏 5.0g 酵母膏 5.0g
蛋白胨 10.0g 葡萄糖 10.0g
乳糖 5.0g NaCl 5.0g
琼脂 20.0g 蒸馏水 1000ml
pH 6.8
145.(Oxoid CM3)
‵Lab-Lemco′beef extract 1g 酵母膏 2g
蛋白胨 5g NaCl 5g
琼脂 15g 蒸馏水 1000ml
pH 7.4
146.综合PDA琼脂(AS 17)
葡萄糖 20.0g KH2PO4 3.0g
MgSO4.7H2O 1.5g 维生素B1 微量
琼脂 15.0g 马铃薯提取液 1000ml


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