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转载:根瘤菌与豆科植物相互关系研究中的新问题

已有 4378 次阅读 2009-10-24 04:54 |个人分类:根瘤菌与豆科植物分子相互作用|系统分类:科研笔记|关键词:根瘤菌,豆科植物,相互关系| 根瘤菌, 相互关系, 豆科植物 |文章来源:转载

下面的问题由Catherine Masson-Boivin等人提出,发表在Trends in Microbiology上,详见:doi:10.1016/j.tim.2009.07.004。值得我们去深思。


1. 根瘤菌具有很大的多样性,能否超出目前的β,α-变形杆菌门?结瘤基因如何起源的?(Does rhizobium diversity extend beyond a- and b-proteobacteria? What is the origin of nod genes? )


2. 不依赖于结瘤因子(NF)的结瘤机制是什么?它与由结瘤因子引起的结瘤这种机制的关系是什么?是否不与NF有关的结瘤机制只局限于合萌属这类植物?自然界中有无其它的结瘤机制呢?What is the actual mechanism for NF-independent nodulation?

How is it related to the NF strategy? Is the NF-independent strategy restricted to Aeschynomene plants? Are there additional nodulation strategies in nature?)


3.根瘤菌的表面多糖在侵染豆科植物过程中的精确任务是什么?它们是不是一些信号的受体?哪一种侵染机制与不依赖于NF的入侵有关?根瘤菌是如何进入根瘤细胞并在其中稳定存在的?( What is the precise role of surface polysaccharides in infection?

Are there receptors for signaling? Which infection mechanisms are associated with NF-independent crack-entry? How are rhizobia internalized and maintained in nodule cells?)


4.如果一个受体基因组接收了nod和nif基因从而进化成根瘤菌,那么这个受体菌的遗传素因究竟是什么?受体和供体基因组之间的系统发育关系究竟是远是近?接收nod和nif的机制是什么呢?(What genetic predisposition should a recipient genome of nod and nif genes display for evolving into a rhizobium? How phylogenetically distant can the recipient and donor genomes be? What are the adaptive mechanisms required?)


5. 共生体的进化是从原始的形式(如不依赖于NF,通过裂隙进入)到精密的方式(依赖NF,有侵染线的形成)?(Has symbiosis evolved from primitive (e.g. NF-independent, crack-entry) to sophisticated (NF, infection thread) strategies?)


6. 在一定时期分化形成类菌体(在山羊豆族的根瘤内)是否是形成固氮器官的必经步骤?(Do the terminally differentiated bacteroids (found in galegoid nodules) constitute a step towards a N2-fixing organelle?)


7. 在结瘤的哪一阶段,T3SS和T4SS分泌效应子?Nops转移到植物胞质是这一功能实施所必须?(At what stages of the nodulation process do T3SS and T4SS secrete effectors? Is translocation of Nops into plant cytoplasm required for their function?)


8. T3SS-和T4SS-依赖的效应子的受体是什么,它们是否能增强或是抑制在特定宿主植物上的结瘤作用?(What are the targets of T3SS- and T4SS-dependent effectors that either enhance or impair nodulation of certain hosts?)




http://blog.sciencenet.cn/blog-3533-264616.html

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