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文新中慢生根瘤菌(Mesorhizobium wenxiniae)

已有 1974 次阅读 2018-4-27 10:36 |个人分类:根瘤菌分类|系统分类:科研笔记| 文新中慢生根瘤菌

张俊杰博士等人最近(2018年4月)在国际系统与进化微生物学杂志(IJSEM)上发表一个分离自鹰嘴豆(chickpea, Cicer arietinum L.)的根瘤菌新物种,定名为:文新中慢生根瘤菌(Mesorhizobium wenxiniae),种名来自陈文新先生的名字的拉丁化,以表示对陈先生的尊重( to honour Wen-xin Chen, a respected soil microbiologist, for her great contributions to the investigation and development of  resources in China)。至此,中慢生根瘤菌属(Mesorhizobium)已有三个物种名字使用了我国根瘤菌研究方面的科学家名字,以纪念他们在根瘤菌分类学研究方面的卓越贡献。除这个物种外,另外两个物种分别是:华癸中慢生根瘤菌(Mesorhizobium huakuii)和庆笙中慢生根瘤菌(Mesorhizobium qingshengii),分别纪念陈华癸和樊庆笙两位根瘤菌研究领域里的先辈。


文新中慢生根瘤菌的发表,使得自我们国家分离的鹰嘴豆根瘤菌新物种增加为2个,另外一个是木垒中慢生根瘤菌(Mesorhizobium muleiense),该物种分离自中国新疆木垒哈萨克自治县,该县的鹰嘴豆种植面积和产量均居国内第一。木垒中慢生根瘤菌物种也由张俊杰博士发表,他现在是国内研究鹰嘴豆根瘤菌分类学、系统学、生态学、进化学及应用方面的首席专家。


世界范围内,自鹰嘴豆中分离的根瘤菌还有地中海中慢生根瘤菌(Mesorhizobium mediterraneum )和鹰嘴豆中慢生根瘤菌(Mesorhizobium ciceri)。因此,我国和国外发表的鹰嘴豆根瘤菌物种数量各占一半。张俊杰等人的研究还发现,国外的两个鹰嘴豆根瘤菌物种在我国并没有发现,说明我国的鹰嘴豆物种的特殊性。国内的这两种特殊鹰嘴豆根瘤菌与国际上发现的另外两个鹰嘴豆根瘤菌物种间的进化关系,生态适应性,共生基因和持家基因的起源、进化、变异,基因组特性等值得深入研究。


鹰嘴豆是药食兼用的豆类植物,研究其与根瘤菌之间的共生固氮关系,有助于我们在种植鹰嘴豆时,接种高效的固氮根瘤菌,从而促进鹰嘴豆的生长,减少氮肥的施用,提高鹰嘴豆的品质和产量。前期研究中,我们还发现,从未种植过鹰嘴豆的地区,土壤内不存在与之相匹配的根瘤菌,而鹰嘴豆对根瘤菌的选择性又十分强,因此,在该地区种植鹰嘴豆时,接种合适的根瘤菌十分必要。


文新中慢生根瘤菌新物种的文章相关信息如下:

Mesorhizobium wenxiniae sp. nov., isolated from chickpea (Cicer arietinum L.) in China.

Zhang J1,2,3, Guo C2, Chen W4, de Lajudie P5, Zhang Z2, Shang Y2, Wang ET6.


Int J Syst Evol Microbiol. 2018 Apr 20. doi: 10.1099/ijsem.0.002770. [Epub ahead of print]


作者单位:

  • 3Collaborative Innovation Center for Food Production and Safety, Henan Province, Zhengzhou 450002, PR China.

  • 1College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan province, 450000, PR China.

  • 2Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Henan province, 450000, PR China.

  • 4College of Biological Sciences and Rhizobium Research Center, China Agricultural University, Beijing 100193, PR China.

  • 5IRD, Univ. Montpellier, CIRAD, INRA, SupAgro, LSTM, Montpellier, France.

  • 6Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México, D. F., Mexico.

Abstract

Three chickpea rhizobial strains (WYCCWR 10195T=S1-3-7, WYCCWR 10198=S1-4-3 and WYCCWR 10200=S1-5-1) isolated from Northwest China formed a group affiliated to Mesorhizobium based on 16S rRNA gene sequence comparison. To clarify their species status, multilocus sequence analysis and average nucleotide identity (ANI) values of whole genome sequences between the novel group and the type strains of the related species were further performed. Similarities of 95.7-96.6 % in the concatenated sequences of atpD-recA-glnII and 91.9-93.1 % of ANI values to the closest-related species Mesorhizobium muleiense, Mesorhizobium mediterraneum and Mesorhizobium temperatum demonstrated the novel group a unique genospecies. The most abundant fatty acid in cells of WYCCWR 10195T were C19 : 0 cyclo ω8c (51.4 %), followed by C18 : 1 ω7c 11-methyl (9.5 %) and C16 : 0 (9.3 %). Its genome size was 6.37 Mbp, comprising 6633 predicted genes with a DNA G+C content of 61.9 mol%. The similarities of 99.0-99.8 % for the nodC gene and 98.3-99.44 % for the nifH gene to those of the chickpea rhizobial species and nodulation with Cicer arietinum L. confirmed the strains of the new genospecies as symbiovar ciceri. The weak utilization of most of the tested sugars/organic acids and non-utilization of l(+)-rhamnose, l-cysteine and l-glycine as sole carbon source, tolerance to 1 % (w/v) NaCl, resistance to 5 μg ml-1 chloromycetin and non-hydrolysis of l-tyrosine distinguished the novel group from the related species and supported this group as a novel species, for which the name Mesorhizobium wenxiniae sp. nov. is proposed, with WYCCWR 10195T (=S1-3-7=HAMBI 3692T=LMG 30254T) as the type strain.

http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002770#tab2 


除了文新中慢生根瘤菌外,其他的细菌也有使用文新的名字,如:

Sphingobacterium wenxinae

Sphingobium wenxiniae

Rhizobium wenxiniae

Paenibacillus wenxiniae

但只有Mesorhizobium wenxiniae真正能反映出与陈先生一生的研究工作有关,即研究能结瘤固氮的根瘤菌。前面的Rhizobium wenxinae,即使带有根瘤菌属(Rhizobium)的名字,但该菌是植物内生菌,不能结瘤固氮。



http://blog.sciencenet.cn/blog-3533-1111163.html

上一篇:根瘤菌,内生菌,根瘤内生菌,根表菌,土壤微生物,根际,Bulk土壤的关系示意图
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