Controls: 1. positive controls: single staining for each of the target proteins using the appropriate label 2. negative controls: omission of one of the primary antibodies but retaining all of the ...
Affinity (亲和力) : the strength of binding of one epitope to a monovalent antibody, such as Fab fragment, and is expressed in terms of the association constant, Ka . 亲和力 为一个抗原表位与单价抗体结合的能力,用 Ka 表示 (M -1 ,1/moles per liter) ,值越大表示 ...