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第一作者:Junzhong Shang
第一单位:华中农业大学
通讯作者:Longqing Chen
Abstract
背景-蜡梅:Wintersweet (Chimonanthus praecox), an important ornamental plant, has evolved unique fragrant aroma and winter-flowering properties, which are critical for its successful sexual reproduction. However, the molecular mechanisms underlying these traits are largely unknown in this species.
背景-系统发育:In addition, wintersweet is also a typical representative species of the magnoliids, where the phylogenetic position of which relative to eudicots and monocots has not been conclusively resolved. 主要研究:Here, we present a chromosome-level wintersweet genome assembly with a total size of 695.36 Mb and a draft genome assembly of Calycanthus chinensis. 结果-系统发育:Phylogenetic analyses of 17 representative angiosperm genomes suggest that Magnoliids and eudicots are sister to monocots. 结果-WGD:Whole-genome duplication signatures reveal two major duplication events in the evolutionary history of the wintersweet genome, with an ancient one shared by Laurales, and a more recent one shared by the Calycantaceae. 结果-花香:Whole-genome duplication and tandem duplication events have significant impacts on copy numbers of genes related to terpene and benzenoid/phenylpropanoid (the main floral scent volatiles) biosynthesis, which may contribute to the characteristic aroma formation. 结果-冬季开花:An integrative analysis combining cytology with genomic and transcriptomic data reveals biological characteristics of wintersweet, such as floral transition in spring, floral organ specification, low temperature-mediated floral bud break, early blooming in winter, and strong cold tolerance. 结论:These findings provide insights into the evolutionary history of wintersweet and the relationships among the Magnoliids, monocots, and eudicots; the molecular basis underlying floral scent biosynthesis; and winter flowering, and highlight the utility of multi-omics data in deciphering important ornamental traits in wintersweet.
摘 要
蜡梅(Chimonanthus praecox)是一种重要的园艺植物,演化出了独特的香气和冬季开花特性,这对于其有性繁殖成功至关重要。然而,蜡梅的这些特性背后潜在的分子机制并不清楚。另外,蜡梅也是木兰类植物的一个代表性物种,而木兰类植物与单、双子叶植物之间的系统发育关系还存在争议。本文中,作者报道了蜡梅染色体级别的参考基因组,总大小约为695.36 Mb,另外还有一个夏蜡梅(Calycanthus chinensis)的基因组草图。通过对17个被子植物代表性物种的系统发育分析显示,木兰类植物与双子叶植物作为姊妹枝,再与单子叶植物聚在一起。全基因组复制信号检测显示在蜡梅的基因组演化过程中共发生过两次全基因组加倍事件,一个是比较古老的樟目共享的全基因组复制事件,还有一次是近期的由蜡梅科物种共享的一次全基因组复制事件。全基因组复制事件与串联重复事件显著增加了与萜烯以及苯类/苯丙烷生物合成相关基因的拷贝数增加,而这些物质可能是蜡梅特有香气形成的一个代谢物质基础。作者进一步通过结合细胞学与基因组、转录组数据的综合分析,揭示了蜡梅的生物学特征,比如在春季发生的成花转变、花器官特化、低温介导的花芽长出以及冬季的早花与超强耐寒特性等。这些研究为蜡梅的演化历史以及木兰类、单子叶植物和双子叶植物之间的系统发育关系提供了新的见解。另外,还有为花香生物合成的分子基础以及和蜡梅冬季开花特性提供了新的见解,并突出了多组学数据在深入解析蜡梅重要观赏性状中的实用性。
通讯作者
**陈龙清** 个人简介: 1981-1985年,华中农业大学,学士; 1985-1988年,华中农业大学,硕士; 1995-1998年,北京林业大学,博士。 研究方向: 园林植物种质资源及其利用、城市植物多样性
doi: 10.1186/s13059-020-02088-y
Journal: Genome Biology
Published date: August 10, 2020
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