主要研究：To comprehensively study and exploit the genome of the neglected oilseed Brassica nigra, we generated two high-quality nanopore de novo genome assemblies.

结果-组装结果：The N50 contig lengths for the two assemblies were 17.1 Mb (12  contigs), one of the best among 324  sequenced plant genomes, and 0.29 Mb (424  contigs), respectively, reflecting recent improvements in the technology.

结果-质量评估：Comparison with a de novo short-read assembly corroborated genome integrity and quantified sequence-related error rates (0.2%). The contiguity and coverage allowed unprecedented access to low-complexity regions of the genome. 

结果-着丝粒特征：Pericentromeric regions and coincidence of hypomethylation enabled localization of active centromeres and identified centromere-associated ALE family retro-elements that appear to have proliferated through relatively recent nested transposition events (<1 Ma).

结果-祖先基因组预测：Genomic distances calculated based on synteny relationships were used to define a post-triplication Brassica-specific ancestral genome, and to calculate the extensive rearrangements that define the evolutionary distance separating B. nigra from its diploid relatives.