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Nature:植物激素信号通路互作组

已有 625 次阅读 2020-7-3 09:30 |个人分类:每日摘要|系统分类:论文交流

Extensive signal integration by the phytohormone protein network

第一作者Melina Altmann

第一单位德国亥姆霍兹慕尼黑中心

通讯作者Pascal Falter-Braun


 Abstract 


背景回顾Plant hormones coordinate responses to environmental cues with developmental programs, and are fundamental for stress resilience and agronomic yield. The core signalling pathways underlying the effects of phytohormones have been elucidated by genetic screens and hypothesis-driven approaches, and extended by interactome studies of select pathways.


提出问题:However, fundamental questions remain about how information from different pathways is integrated. Genetically, most phenotypes seem to be regulated by several hormones, but transcriptional profiling suggests that hormones trigger largely exclusive transcriptional programs.


提出假设:We hypothesized that protein–protein interactions have an important role in phytohormone signal integration.


主要工作:Here, we experimentally generated a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein–protein interactions.


结果1~蛋白-蛋白互作:In the highly interconnected network, we identify pathway communities and hundreds of previously unknown pathway contacts that represent potential points of crosstalk. Functional validation of candidates in seven hormone pathways reveals new functions for 74% of tested proteins in 84% of candidate interactions, and indicates that a large majority of signalling proteins function pleiotropically in several pathways.


结果2~激素-受体互作:Moreover, we identify several hundred largely small-molecule-dependent interactions of hormone receptors. Comparison with previous reports suggests that noncanonical and nontranscription-mediated receptor signalling is more common than hitherto appreciated.


 摘  要 


植物激素响应于环境因素来协调植物的发育进程,并且对于胁迫适应和农艺产量非常重要。通过遗传筛选和假设驱动的方法,研究者们已经鉴定了这些激素发挥作用的核心信号通路,并且通过对于特定信号通路的互作组拓宽了我们对于这些通路的了解。然而,不同通路上的信号是如何在植物体内整合的还不清楚。从遗传上来说,大多数的表型受到少数的几个激素调控,但是转录图谱却显示激素诱导了各自特异性的转录编程。作者假设蛋白-蛋白互作在激素信号整合中发挥重要作用。本文中,作者通过实验生成了拟南芥激素信号网络的系统级图谱,包含2,000多种二元的蛋白-蛋白互作。在高度互连网络中,作者鉴定了通路共同体以及数百个之前未曾发现的通路关联,这些可能是潜在的通路交联点。作者选择了7个激素通路上的候选互作关系进行验证,结果显示84%的候选互作关系中,74%测试蛋白具有新的功能,表明大多数的信号转导蛋白在多个通路中具有多效性。此外,作者还鉴定了数百个激素受体的小分子依赖型互作。通过与先前的研究比较显示非经典的和非转录介导的受体信号转导要比我们迄今认识到的更加普遍。


 通讯作者 


**Pascal Falter-Braun**


研究方向:

大分子网络如何控制生物进程以及网络演化


doi: 10.1038/s41586-020-2460-0


Journal: Nature

Published date: July 01, 2020



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