Plant cell-cell transport via plasmodesmata is regulated by light and the circadian clock

First author: Jacob O Brunkard; Affiliations: University of California, Berkeley (加利福尼亚大学伯克利分校): Berkeley, USA

Corresponding author: Patricia C. Zambryski

Plasmodesmata (PD) are essential for plant development, but little is known about their regulation. Several studies have linked PD transport to chloroplast-centered signaling networks, but the physiological significance of this connection remains unclear. Here, we show that PD transport is strongly regulated by light and the circadian clock. Light promotes PD transport during the day, but light is not sufficient to increase rates of PD transport at night, suggesting a circadian gating mechanism. Silencing expression of the core circadian clock gene, LHY/CCA1, allows light to strongly promote PD transport during subjective night, confirming that the canonical plant circadian clock controls the PD transport light response. We conclude that PD transport is dynamically regulated during the day/night cycle. Due to the many roles of PD in plant biology, this discovery has strong implications for plant development, physiology, and pathogenesis.

胞间连丝对于植物的发育至关重要，但目前我们对于胞间连丝的调控机制了解甚少。一些研究将胞间连丝转运和以叶绿体为中心的信号转导网络联系到一起，但这种关联潜在的生理意义仍不明确。本文，作者的研究显示胞间连丝转运受到光和生物钟的强烈调控。光会促进白天胞间连丝转运，但不能增加晚上胞间连丝转运的速率，说明存在一个生物钟开关。沉默一个核心生物钟基因LHY/CCA1的表达使得光在晚上也能促进胞间连丝转运，说明植物的生物钟的确控制着胞间连丝转运对于光的响应。本文的研究揭示了胞间连丝转运在日夜周期循环中受到动态调控。由于胞间连丝转运在植物生物学中的多方面作用，本文的研究对于植物发育、生理以及病理学均具有非常重要的意义。

通讯：Patricia C. Zambryski (https://plantandmicrobiology.berkeley.edu/profile/zambryski)

个人简介：1969年，麦吉尔大学，遗传学学士；1974年，科罗拉多大学，分子生物学博士。

研究方向：农杆菌介导的植物转基因系统；植物细胞间通过胞间连丝进行交流的机制。

doi: https://doi.org/10.1104/pp.19.00460

Journal: Plant Physiology

Published date: October 10, 2019