Updates in nanopore technology (纳米孔技术) have made it possible to obtain gigabases of sequence data. Prior to (在…之前) this, nanopore sequencing technology was mainly used to analyze microbial samples (微生物样品). Here, we describe the generation of a comprehensive nanopore sequencing dataset with a median read length of 11,979 bp for a self-compatible accession of the wild tomato species Solanum pennellii. We describe the assembly of its genome to a contig N50 of 2.5 MB. The assembly pipeline comprised initial read correction with Canu and assembly with SMARTdenovo. The resulting raw nanopore-based de novo genome is structurally highly similar to that of the reference S. pennellii LA716 accession but has a high error rate and was rich in homopolymer (均聚物) deletions. After polishing (修正) the assembly with Illumina reads, we obtained an error rate of <0.02% when assessed versus the same Illumina data. We obtained a gene completeness of 96.53%, slightly surpassing (优于) that of the reference S. pennellii. Taken together our data indicate that such long read sequencing data can be used to affordably sequence and assemble gigabase-sized plant genomes.

研究方向：植物科学，包括植物对不同的糖水平的反应，植物如何将碳整合到细胞壁中以及这些壁如何被降解和利用等。

First Published data: October 12, 2017.