TickingClock的个人博客分享 http://blog.sciencenet.cn/u/TickingClock

博文

Plant Cell:GWAS应用于拟南芥的代谢QTL定位

已有 2270 次阅读 2017-9-30 08:40 |个人分类:每日摘要|系统分类:论文交流

Genome-wide Association Mapping Reveals that Specific and Pleiotropic Regulatory Mechanisms Fine-tune Central Metabolism and Growth in Arabidopsis


First author:Corina M. Fusari; Affiliations: Max Plank Institute of Molecular Plant Physiology (分子植物生理学马克斯普朗克研究所): Potsdam-Golm, Germany

Corresponding author: Mark Stitt; Joost J.B. Keurentjes


Central metabolism (中心代谢) is a coordinated network that is regulated at multiple levels by resource availability (资源可用性) and by environmental and developmental cues. Its genetic architecture has been investigated by mapping metabolite quantitative trait loci (QTL). A more direct approach is to identify enzyme activity QTL, which distinguishes (区别) between cis-QTL in structural genes encoding enzymes and regulatory trans-QTL. Using genome-wide association studies, we mapped QTL for 24 enzyme activities, 9 metabolites, 3 structural components, and biomass in Arabidopsis thaliana. We detected strong cis-QTL for five enzyme activities. A cis-QTL for UDP-glucose pyrophosphorylase (UDP-葡萄糖焦磷酸化酶) activity (UGP) in the UGP1 promoter is maintained through balancing selection (平衡选择). Variation in acid invertase (酸转化酶) activity (aINV) reflects multiple evolutionary events in the promoter and coding region of VAC-INV. Cis-QTL were also detected for ADP-glucose pyrophosphorylase (AGP), fumarase (FUM; 延胡索酸酶), and phosphoglucose isomerase (磷酸葡糖异构酶) activity (PGI). We detected many trans-QTL, including transcription factors, E3 ligases (E3连接酶), protein targeting components, and protein kinases and validated some by knockout analysis. Trans-QTL are more frequent but tend to have smaller individual effects than cis-QTL. We detected many co-localized QTL, including a multi-trait QTL on chromosome 4 that affects six enzyme activities, three metabolites, protein, and biomass. These traits are coordinately modified by different ACCELERATED CELL DEATH6 alleles (ACD6等位基因), revealing a trade-off (权衡) between metabolism and defense against biotic stress.


中心代谢是一个协调的网络,受到资源可用性、环境和发育等多个水平的调控。其遗传结构已经有报道通过定位代谢QTL进行研究。更加直接的方法是通过鉴定酶活QTL,区分开编码酶的结构基因的顺式QTL和调控作用的反式QTL。利用全基因组关联分析,本文定位了拟南芥中24个酶活、9个代谢、3个结构组分和生物量相关的QTL。作者鉴定了5个与酶活强相关的顺式QTL。UGP1启动子上的一个顺式QTL与UDP-葡萄糖焦磷酸化酶活相关,其通过平衡选择维持。酸转化酶活的变异反映了在VAC-INV启动子和编码区经历的多次进化事件。本文还鉴定了ADP-葡萄糖焦磷酸化酶、延胡索酸酶、磷酸葡糖异构酶活相关的顺式QTL。作者还鉴定了包括转录因子、E3连接酶、蛋白寻靶组分蛋白激酶相关的反式QTL,并验证了一些通过敲除试验得到的QTL。反式QTL更加普遍,但其单个的效应要比顺式QTL要小。作者还鉴定到了多个共定位的QTL,包括在4号染色体上的多位点QTL,该QTL影响6个酶活、3个代谢物、蛋白及生物量。这些位点被不同的ACD6等位基因所协调修饰,揭示了在代谢与生物胁迫抗性之间的平衡。


通讯:Mark Stitt (http://www.mpimp-golm.mpg.de/5728/dep_2)


研究方向:植物光合碳代谢、氮磷利用和生长。


doi: https://doi.org/10.1105/tpc.17.00232


Journal: the Plant Cell
First Published data: September 27, 2017.

P.S. 欢迎关注微信公众号:微信号Plant_Frontiers


http://blog.sciencenet.cn/blog-3158122-1078444.html

上一篇:Tree Physiology:PtoWOX5a参与杨树中不定根的发育
下一篇:Nature Plants:植物细胞凋亡(Perspective)

0

该博文允许注册用户评论 请点击登录 评论 (0 个评论)

数据加载中...

Archiver|手机版|科学网 ( 京ICP备14006957 )

GMT+8, 2019-12-15 20:48

Powered by ScienceNet.cn

Copyright © 2007- 中国科学报社

返回顶部