第一步：建库
使用命令diamond makedb --in 建库文件.fa --db 输出文件名称
E:\Util_file\diamond-windows>diamond makedb --in Brassica_napus.annotation_v5.pep_rename.fa --db Bra
ssica  #建库示例命令
diamond v0.9.32.133 (C) Max Planck Society for the Advancement of Science
Documentation, support and updates available at http://www.diamondsearch.org

#CPU threads: 8
Scoring parameters: (Matrix=BLOSUM62 Lambda=0.267 K=0.041 Penalties=11/1)
Database input file: Brassica_napus.annotation_v5.pep_rename.fa
Opening the database file...  [0s]
Loading sequences...  [0.382s]
Masking sequences...  [0.624s]
Writing sequences...  [0.116s]
Hashing sequences...  [0.015s]
Loading sequences...  [0s]
Writing trailer...  [0.007s]
Closing the input file...  [0s]
Closing the database file...  [0s]
Database hash = 349da09b950d300f557b96e24122a623
Processed 101040 sequences, 33719134 letters.
Total time = 1.156s

E:\Util_file\diamond-windows>diamond blastp --db Brassica -q Gmax_508_Wm82.a4.v1.protein_primaryTran
scriptOnly.fa -o BrassicaWithGmax.txt  #比对实例命令
diamond v0.9.32.133 (C) Max Planck Society for the Advancement of Science
Documentation, support and updates available at http://www.diamondsearch.org

#CPU threads: 8
Scoring parameters: (Matrix=BLOSUM62 Lambda=0.267 K=0.041 Penalties=11/1)
Temporary directory:
Opening the database...  [0s]
#Target sequences to report alignments for: 25
Reference = Brassica.dmnd
Sequences = 101040
Letters = 33719134