Abstract

Intracellular reactive sulfur species and reactive oxygen species play vital roles in immunologic mechanism. As an emerging signal transmitter, SO₂ can be generated as the anti-oxidant, while SO₂ is also a potential oxidative stress-inducer in organism. Aiming to elucidate in-depth the dichotomous role of SO₂ under oxidative stress, we designed a dual-response fluorescent probe that enabled the respective or successive detection of SO₂ and ClO⁻. The probe itself emits the red fluorescence (625 nm) which can largely switch to blue (410 nm) and green fluorescence (500 nm) respectively in response to SO₂ and ClO⁻, allowing the highly selective and accurate ratiometric quantification for both SO₂ and ClO⁻ in cells. Moreover the ultrafast (SO₂: <60 s; ClO⁻: within sec) and highly sensitive (detection limits: SO₂: 3.5 nM; ClO⁻: 12.5 nM) detection were achieved. With the robust applicability, the developed probe was successfully used to quantify SO₂ and endogenous ClO⁻ in respectively the HeLa cells and the RAW 264.7 cells, as well as to visualize the dynamic of SO₂/ClO⁻ in zebrafish. The fluorescent imaging studies and flow cytometry analysis confirmed the burst-and-depletion and meanwhile the oxidative-and-antioxidative effects of intracellular SO₂ under the NaClO induced oxidative stress.

A novel dual-ratiometric-responsefluorescent probe for SO2/ClO− detection in cells and in vivo and itsapplication in exploring the dichotomous role of SO2 under the ClO− inducedoxidative stress

Kun Dou, Qiang Fu, Guang Chen, Fabiao Yu Yuxia Liu,Ziping Cao, Guoliang Li, Xianen Zhao, Lian Xia, Lingxin Chen, Hua Wang, JinmaoYou

Biomaterials Volume 133, July 2017, Pages82–93

http://www.sciencedirect.com/science/article/pii/S0142961217302545