摘 要:多硫化氢（H₂S_n）和亚硝酰氢（HNO）在一系列生理病理过程中起着重要的作用，包括调节细胞内氧化还原信号传递过程、增强心肌的收缩能力，抑制血小板聚集等。H₂S_n可以通过硫化氢（H₂S）与活性氧物种反应得到。一氧化氮（NO）和HNO可以在超氧化物歧化酶（SOD）作用下相互转化，H₂S和NO反应可以生成H₂S_n和HNO，调控酶的活性以及蛋白与蛋白之间的相互作用，从而影响蛋白质的生理功能。因此，实时检测生物体内H₂S_n和HNO的浓度具有十分重要的生物医学意义。在各种生物检测技术中，荧光探针具有选择性好，灵敏度高，可以实时原位检测，对样品损伤小等优点，受到了广泛关注。本文将按照探针响应基团的反应类型，将近几年用于定性定量检测H₂S_n和HNO荧光探针进行分类和总结，重点概述探针的设计理念、响应机制和生物应用，并对探针的应用前景进行了展望。同时，本文也关注了硫化氢和其他硫烷硫类物种荧光检测的近期进展。

关键词: 荧光探针; 多硫化氢；亚硝酰氢；硫化氢；硫烷硫；荧光成像

doi：10.7517/j.issn.1647-0475.2016.05.0402

文章编号：1647-0475(2016)05-0402-24

用于检测多硫化氢和亚硝酰氢的小分子荧光探针

张鹏，韩潇玥，于法标*，陈令新*

影像科学与光化学, 2016,34（5），402-425.

http://www.yxkxyghx.org/CN/abstract/html/20160502.htm

Small-molecular Fluorescent Probes for the Detection of HydrogenPolysulfides and Nitroxyl

ZHANG Peng, HAN xiaoyue, YU Fabiao,* CHEN Lingxin*

Imaging Science and Photochemistry，2016,34（5），402-425.

Abstract: Hydrogenpolysulfides (H₂S_n) and nitroxyl (HNO) play importantroles in a series of physiological and pathological processes, includingregulating intracellular redox signaling processes, strengthening myocardialcontractility and inhibiting platelet aggregation. H₂S_ncan be formed by the reaction between hydrogen sulfide (H₂S) andreactive oxygen species. Nitric oxide (NO) and HNO can interconvert in thepresent of superoxide dismutase. H₂S can react with NO and form H₂S_nand HNO, which will alter the enzymatic activity and protein–proteininteractions, and then affect protein functions. Therefore, the capability toreal-time detect the concentrations of H₂S_n and HNO invivo are of great significance in biomedical research area. Among the variousbioanalysis methods, the fluorescent probe technology with the advantages ofgood selectivity, high sensitivity, real-time in situ detection and negligibledamage to sample, has received widespread attention. This review classifies andsummarizes the fluorescent probes which are developed in recent years to detectH₂S_n and HNO concentrations based on the reaction typesbetween the response units and the analytes. This review mainly focus onelaborating the design concepts, detection mechanisms and biologicalapplications of these probes. Finally, the application prospect of fluorescentprobes are discussed. At the same time, this review also cover the fluorescenceprobes for the detection of hydrogen sulfide and other sulfane sulfur.

Key words: fluorescent probe; hydrogen polysulfides;nitroxyl; hydrogen sulfide; sulfane sulfur; fluorescent imaging