博文
- Tubes are made with polypropylene
- Before running ELISA and LegendPlex, make sure the microcentrifuge tubes and pipette tips are made of polypropylene. Data are expressed as mean ± SD from at least three independent experiments with triple replicates per experiment. (* p 0.05, ** p 0.01, *** p &nbs ...
- Transfection vs. Transduction vs. Transformation
- 转化、转染、转导、感染有什么区别？ 涉及外源基因表达的四个词：转化、转染、转导、感染，可是与实验狗们天天相伴的几个概念。与他们相处了这么久，但是你清楚他们之间的区别吗？今天小赛就带你重新梳理下这四个词，快来看看你用对了吗？ 转化Transformation： 是指含外源基因的重组质粒（载体）将外源基 ...
- Acetic acid (醋酸或乙酸) 溶液的配制
- 问：如何配制1M的acetic acid溶液？ 答：先计算母液的浓度。0.99 （purity）* 1.049 g/mL (density) / 60.05 g/ m ol ( molecular weight ) =0.0173 mol/mL = 13.7 mol/L = 17.3 M 所以，1M acetic acid溶液是将母液稀释17.3倍得到的，即1mL母液溶于16.3 mL dH2O中。
- LC-MS
- Liquid chromatography-massspectrometry has been used to detect the 3 different change levels. 1. Drug concentration 2. Drug metabolites (metabolomics) 3. Proteinmics (the protein level changes due to drug intake) Sam runs the University of Chicago Core of ...
- Seahorse notes
- Oligomycin: a complex V inhibitor, derive ATP-linked respiration and proton leak respiration. FCCP: ie. proton (H + ), for the maximum capacity ofH + Rotenone: shut down ETC (electron transport chain) function, revealing the non-mitochondrial respiration.non-mutdrome consumptio ...
- Immune cell markers
- CD3: Universal T cells CD4: helper T cells CD8: cytotoxic T cells CD19: B cells CD21: mature B cells/ follicular dendritic cells CD25: T regulatory cells FoxP3: T regulatory cells (more specific) IFN: interferon cytokines
- Cell death markers
- Bcl-2 superfamily Pro-apoptotic Bax, Bid, Bok, Bik, Bak, Noxa, Bim, Puma, Bad Anti-apoptotic BCL-2, A1, BCL-XL, BCL-W, BLFL-1, BCL-B, MCL-1 Caspases Initiator: Caspase 2,Caspase 8,Caspase 9,Caspase 10 Effector:Caspase 3,Caspase 6,Casp ...
- glutamine vs. glutamate
- glutamine vs. glutamate (glutamate =L-Glutamic acid ) 谷氨酰胺vs.谷氨酸 它们是不同的氨基酸 Glutamine （谷氨酰胺）is a conditionally essential amino acid that has various functions of the body. Glutamate （谷氨酸） is a non-essential amino acid whic ...
